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- Procedure: analytically transfer between 0.4053g of iron(III) chloride hexahydrate, FeCl 3•6 H2O (M = 270.2957g•mol-1), and dissolve in 5.5 mL distilled, deionized water. Support the tube on a stir plate and over a 15-minute period add, using the 10mL burette, 5mL of a nominal 600mM solution of 2,4 pentandione in methanol under constant stirring. To the resulting solution, add via a 10mL burette 3mL of a nominal 450mM sodium acetate solution stirring constantly. At this point, a red crystalline solid should begin to precipitate. Transfer and support the reaction vessel in the hot water bath and once the reaction mixture has reached a stable temperature in the desired range, maintain the reaction mixture at this temperature, under constant stirring, for a 15-minute period Initial weight of iron(III) chloride hexahydrate: 0.4053g The final weight of the reaction tris(acetylacetonate)iron(III): 19.6 mg 3. What is the charge on the metal in the starting material for your synthesized…For Indirect Iodometric Analysis of Copper... ~0.0896g KIO3 necessary to consume 350mL of 0.1 M Na2S2O3, Na2S2O3 is stored in an amber glass bottle until ready for use. Primary Standard KIO3 has 2g of KI, 50mL of DI water, and 10 mL of 1.0M HCl is added then immediately titrated with Na2S2O3 until medium yellow or straw... then 5mL of starch indicator is added and titrated again until blue black color turns clear. Unknown CuO use 1.2G of Unknown, 20mL of HNO3 heated until sample dissolved, 25 mL of DI water added and boiled until clear light blue color, after cooling 1:1 NH3 added (~34.47 mL of NH4OH reagent) until permanent deep blue color amine complex, 2g of NH4HF2 added and swirled until dissolved, 3 g of KI is added then titrated immediately with Na2S2O3 until brown color of iodide is nearly gone (brown milk color), 2 g of KSCN and 3 mL of starch indicator is then added with titration continuing until disappearance of new blue black color. 1. Na2CO3 is often added to thiosulfate…For Indirect Iodometric Analysis of Copper... ~0.0896g KIO3 necessary to consume 350mL of 0.1 M Na2S2O3, Na2S2O3 is stored in an amber glass bottle until ready for use. Primary Standard KIO3 has 2g of KI, 50mL of DI water, and 10 mL of 1.0M HCl is added then immediately titrated with Na2S2O3 until medium yellow or straw... then 5mL of starch indicator is added and titrated again until blue black color turns clear. Unknown CuO use 1.2G of Unknown, 20mL of HNO3 heated until sample dissolved, 25 mL of DI water added and boiled until clear light blue color, after cooling 1:1 NH3 added (~34.47 mL of NH4OH reagent) until permanent deep blue color amine complex, 2g of NH4HF2 added and swirled until dissolved, 3 g of KI is added then titrated immediately with Na2S2O3 until brown color of iodide is nearly gone (brown milk color), 2 g of KSCN and 3 mL of starch indicator is then added with titration continuing until disappearance of new blue black color. 4. Why is the starch indicator solution…
- If given the option between using a P1000 and a P200 pipetter to dispense 200 uL, the P200 should be chosen as it can pipette that amount with greaterSuppose you have a soil that is made up of 10% organic matter with CEC = 200 cmol/kg, 40% kaolinitewith CEC = 10 cmol/kg, and 50% vermiculite with CEC = 100 cmol/kg.1- Calculate the overall CEC of the soil by taking a weighted average of the three soil components.2- What percentage of the overall CEC is contributed by the organic matter? How does that numbercompare to the 10% of the soil itself that is organic?3. Assume that in extraction from water into toulene, analyte A has distribution ratio of 10. A 20 mL portion of an aqueous solution of A is extracted with toulene. Which of thw following procedures will result in most efficient removal of A from the aqueous phase into toulene? (Please show the solution of the correct answer) A. 1 extraction with 20 mL of touleneB. 1 extraction with 40 mL of touleneC. 2 extractions with 20 mL of toulene eachD. 4 extractions with 10 mL of toulene each
- A brewed tea brand sample was subjected to solvent extraction to extract caffeine. The cup of tea measuring 200 mL was extracted with petroleum ether. With KD = 20.0 (caffeine in pet ether-water), the % caffeine (194.10) extracted after 3 extractions with a total of 75.0 mL pet ether used is: A. 97.7% B. 28.6% C. 71.4% D. 2.33%As a newly appointed research officer, Marissa has been assigned a task to separate a mixture containing acetone, acetamide and 1,2-dichloroethane using a high-performance liquid chromatography (HPLC) technique. The instrument was equipped with a 40-cm column packed with cyano propyl bonded to the siloxane backbone material. i) List THREE (3) examples of mobile phase solvent/solvent systems that can be used with the column above. ii) Determine the sequence in which the three analytes eluted from the column and explain the underlying principle applied. iii) Sketch the chromatogram that would be produced if the column was replaced with a column filled with C18 hydrocarbon bound to the siloxane backbone material and acetonitrile was employed as the mobile phase. Label the peaks observed.The concentration of purified OXA-M290 is tested with a BCA assay. Serial dilutions of a bovine serum albumin (BSA) stock solution are prepared, then pipetted into a 96-well plate; each dilution of the BSA standard is tested in triplicate. Then, bicinchoninic acid and Cu2+ ions are added to all of the wells of the plate. After incubating the plate for 1 hour, a microplate reader is used to measure the absorbance of all of the wells in the plate at 560 nm. This generates the following data: BSA conc. (μg/mL), Replicate 1 Absorbance, Replicate 2 Absorbance, Replicate 3 Absorbance 40, 1.360, 1.403, 1.481 20, 0.750, 0.745, 0.810 10, 0.380, 0.344, 0.398 5, 0.198, 0.160, 0.183 2.5, 0.090, 0.100, 0.085 1.25, 0.038, 0.043, 0.051 0.625, 0.024, 0.028, 0.019 Prepare a calibration curve using these data. You can use Excel, R, SPSS or an equivalent graphing software. In this graph, plot absorbance (y-axis) against the concentration of the protein standard (x-axis). Calculate and plot…
- How would you prepare 1ml of a 0.5wt% alginate hydrogel with 30mg/ml BSA from stock solutions of 1wt% alginate in water, 300mg/ml BSA in water, and plain water? I know I need to use the formula C1V1=C2V2 but I am stuck. I need to figure out how much 1wt% alginate, BSA, and plain water I need to use to make the solution.Solution Calculations Using unit analysis, clearly show the set-ups for your calculations. You wish to prepare 50.0 mL of 2.30 x 10–2 M solution of Levothyroxine (hint-how many grams will you need of Levothyroxine?) 1. Describe how to prepare this solution from the pure drug and deionized water. Analytical balances and volumetric flasks are available to you. (Assume that Levothyroxine dissolves in water, at least to the extent necessary for this problem.) Use complete sentences in your description of the solution preparation process. a. A patient is given 2.0mL of a 3.00% (m/v) solution of Levothyroxine, how many milligrams of the drug did they receive? b. What is the molarity of a 3.00% (m/v) solution of Levothyroxine?how much iron ore (that assays at 33.8% fe) must be used in an analytical procedure that is known to have a low bias of -0.15g if an overall relative error of 2.09 ppth in the iron content is desired