Experiment 1: Does Enzyme Concentration affect Amylase Activity? To investigate the effect of enzyme concentration on amylase activity, we mixed 6 ml of 0.5% amylose solution with 70ul of amylase enzyme solution. Each trial used a different concentration of enzyme solution. At 15 second intervals, we tested the reaction mixture for the presence of amylose starch by dropping the mixture into a well containing a drop of Lugol's iodine. We compared our results against the following: a well that contained only amylase solution and iodine, a well containing only enzyme solution and iodine, and a well containing completely digested (or broken-down) starch solution and iodine. We recorded the time of completion of the reaction for each of our trials, when no more starch was present, as the first sample that did not show a positive starch reaction.
Experiment 1: Does Enzyme Concentration affect Amylase Activity? To investigate the effect of enzyme concentration on amylase activity, we mixed 6 ml of 0.5% amylose solution with 70ul of amylase enzyme solution. Each trial used a different concentration of enzyme solution. At 15 second intervals, we tested the reaction mixture for the presence of amylose starch by dropping the mixture into a well containing a drop of Lugol's iodine. We compared our results against the following: a well that contained only amylase solution and iodine, a well containing only enzyme solution and iodine, and a well containing completely digested (or broken-down) starch solution and iodine. We recorded the time of completion of the reaction for each of our trials, when no more starch was present, as the first sample that did not show a positive starch reaction.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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What would be the control variables if any and the controls use in this experiment?
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experiments. You will need to clearly define the controls, the independent variable, and the dependent
variable.
Experiment 1: Does Enzyme Concentration affect Amylase Activity?
To investigate the effect of enzyme concentration on amylase activity, we mixed 6 ml of 0.5% amylose
solution with 70ul of amylase enzyme solution. Each trial used a different concentration of enzyme solution.
At 15 second intervals, we tested the reaction mixture for the presence of amylose starch by dropping the
mixture into a well containing a drop of Lugol's iodine. We compared our results against the following: a
well that contained only amylase solution and iodine, a well containing only enzyme solution and iodine,
and a well containing completely digested (or broken-down) starch solution and iodine. We recorded the
time of completion of the reaction for each of our trials, when no more stareh was present, as the first sample
that did not show a positive starch reaction.
2.
Should enzyme concentration affect the time it takes the reaction to reach completion? Why?
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