Use the plot (below) to estimate values of KM and Vmax for an enzyme-catalyzed reaction. Use 1 significant figure. 40 Vo (uM - s) 20 10 15 20 [S] (uM) Vmax is approximately v uM·s-1. KM is approximately µM.

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Q: [S, mmol L-') Vo (umol L-' min-') 0.5 50 1.0 2.0 3.0 10.0 8||||

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Q: kąk4 k2k1 E +S → ES → E + P (Vmax,f\ rax.f (Vmax,b [P] KM.f KM.b [S] [P] Vo 1+ KM.f Кмь KM,b ||

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