Experiment Title: Cytotoxicity TestExperiment no. 2Objectives: Study the eytotoxicity and how it is measured.1- BackgroundCytotoxicity can lead healthy living cells to three potentialcellular fates.1. Neerosis (accidental cell death): Rapid loss of membrane integrity and celllysis2. Apoptosis (programmed cell death): slower, more orderly, and geneticallycontrolled3. Cytostasis (a decrcase in cell viability): cells remain alive but fail toactively grow and divideImportance of measuring cytotoxicity two major reasons1. Either youwant specific cells to dielook for an adequacompound/condition (cancer and immotherapy)2. Or you want to exclude cyto icity in specific cells (chemicals and drugs)1 have a report on thissubject, please solveApplications- Drug discovery proces- Oncological research- Safety evaluation of pesti ides, plantextracts, food additives, cosmetics, andmy topic.industrial chemicalsmyClassification of cytotoxicity and cell viability assaysThese essays are classified according to measurement types of endpoints(color changes, fluorescence, luminescent, etc.).1. Dye exclusion: Trypan blue, cosin, Congo red, erythrosine B assays.2. Colorimetric assays: MTT assay, MTS assay, XTT assay, WST-I assay,WST-8 assay, LDH assay, SRB assay. NRU assay, and crystal violet assay.3. Fluorometric assays: alamarBlue assay and CFDA-AM assay.4. Luminometric assays: ATP assay and real-time viability assay.2- Experimental Procedure1- Plate ceils into 96-well tissue culture plates. In general, 5000 - 10,000 cellsper well according to the cxperimental factors tested. Carry out yourexperiment by adding chemicals or biological agents into the appropriatewell. Incubate for 6 to 24 hours.2- remove 96 well plates from the incubator and add 20 ul MTT stocksolution to each weil3- place 96 well into an incubator at 37°C, 5% CO2 for 4 hours4- remove 96 well plates fiomn the incubator and aspirate the solution5- add 100 ul DMSO to each well to dissolve the formazan6- rotate the plate for 5 min to distribute eveniy7- Record absorbance at 540nmFigure 1. Cytotoxicity test on 96 well plates that was monitored by ELISA reader3- Discussion1- Explain another methods by which cytotoxicity is measured ?

Cytotoxicity assays measure the ability of cytotoxic compounds to cause cell damage or cell death.…

Experiment Title: Cytotoxicity Test Experiment no. 2 Objectives: Study the eytotoxicity and how it is measured. 1- Background Cytotoxicity can lead healthy living cells to three potential cellular fates 1. Necrosis (accidental cell death): Rapid loss of membrane integrity and cell lysis 2. Apoptosis (programmed cell death): slower, more orderly, and genetically controlled 3. Cytostasis (a decrease in cell viability): cells remain alive but fail to actively grow and divide Importance of measuring cytotoxicity two major reasons 1. Either you want specific cells to die lok for an adequa compound/condition (cancer and immotherapy) 2. Or you want to exclude cyto icity in specific cells (chemicals and drugs) have a report on this subject, please solve my topic. Applications - Drug discovery proces 1 Oncological research - Safety evaluation of pesti ides, plantextracts, food additives, cosmetics, and industrial chemicals

Experiment Title: Cytotoxicity Test Experiment no. 2 Objectives: Study the eytotoxicity and how it is measured. 1- Background Cytotoxicity can lead healthy living cells to three potential cellular fates 1. Necrosis (accidental cell death): Rapid loss of membrane integrity and cell lysis 2. Apoptosis (programmed cell death): slower, more orderly, and genetically controlled 3. Cytostasis (a decrease in cell viability): cells remain alive but fail to actively grow and divide Importance of measuring cytotoxicity two major reasons 1. Either you want specific cells to die lok for an adequa compound/condition (cancer and immotherapy) 2. Or you want to exclude cyto icity in specific cells (chemicals and drugs) have a report on this subject, please solve my topic. Applications - Drug discovery proces 1 Oncological research - Safety evaluation of pesti ides, plantextracts, food additives, cosmetics, and industrial chemicals

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter14: Formula Method

Section: Chapter Questions

Problem 40SST

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EXPERIMENT: CRYOPRESERVATION OF CULTURED CELL LINES Procedure for Cryopreserving MCF-7 and THP-1 Cells• Ensure that the culture is up to the late log phase (assumption is made based on the cell concentration). Prepare the cell suspension and determine the cell concentration.• Dilute the cell suspension in 1:1 ratio with freezing medium (final concentration approximately 1 X 106 – 1 X 107cells/ml is desired). • Dispense the cell suspension into prelabeled cryovial and place the vial in -80°C freezer overnight (temporary storage).• Transfer the cryovial into liquid nitrogen cryotank for long term storage the following day.• After day 3, determine the cell concentration in the freezing medium andcompare with that of the stock concentration. Question: Please briefly explain what is cryopreservation(1), how procedure above are useful to make this experiment successful(2)
Compare the lysis for bacteria to that for CHO (Chinese Hamster Ovary) cells. Note, CHO cells are commonly used for production of therapeutics. How resistant are CHO cells compared to the bacterial cells? If the same level of disruption is expected for CHO cells and the homogenizer is operated at the same pressure for both types of cells, would you expect CHO cells to require more or less number of passes compared to E.coli or Bacillis brevis cells?
describe the steps involved in primary cell dissociation protocol, assessing cell viability and sub culturing adherent cells with trypsin.
Liquid supported membrane of DGA used for rare earth elements separation. Tell me the background/introduction of this research, current state of the art, motivation, how these membrane are made , what is the appropriate characteristics of them, approaches that author followed, you can describe the problems of that approaches, why rare earth is challenging to separate, why current state of the art do not address that need, include two or 3 results and describe it. take those information from few journal papers and mention the correct citation.
(a) Insert the table with data for Experiment 1 Table 1. Osmosis in a model cell contents of dialysis bag contents of beaker mass at 0 min (g) mass at 15 min (g) mass at 30 min (g) mass at 45 min (g) 1 2% sucrose 2% sucrose 11.66 11.75 11.67 11.73 2 20% sucrose 2% sucrose 12.54 12.72 12.98 13.21 3 2% sucrose 20% sucrose 12.11 11.86 11.59 11.48 (b) Calculate the total change in volume with the appropriate units for each dialysis bag in the experiment. Bag 1: Bag 2: Bag 3: (c) Explain why the volume of each bag changed during the experiment.
Experiment No. 1. Five rabbits were inoculated in the right lung and in the left side of the neck with five minims of sterilized water in which was suspended a sufficient quantity of a pure culture (third generation) of the tubercle bacillus to render the liquid quite perceptibly turbid The needle of the Koch’s inoculating syringe was inserted subcutaneously on the left side of the neck and in the third intercostal space to a depth of thirty millimetres on the right side. These animals were then confined in a small box and put in a dark cellar. They were thus deprived of light, fresh air and exercise and were also stinted in the quantity of food given them while being themselves artificially infected with the tubercle bacillus. Experiment No. 2. Five healthy rabbits were placed under the following conditions: A fresh hole about ten feet deep was dug in the middle of a field, and the animals having been confined in a small box with high sides but no top, were lowered to the bottom of…
Experiment No. 1. Five rabbits were inoculated in the right lung and in the left side of the neck with five minims of sterilized water in which was suspended a sufficient quantity of a pure culture (third generation) of the tubercle bacillus to render the liquid quite perceptibly turbid The needle of the Koch’s inoculating syringe was inserted subcutaneously on the left side of the neck and in the third intercostal space to a depth of thirty millimetres on the right side. These animals were then confined in a small box and put in a dark cellar. They were thus deprived of light, fresh air and exercise and were also stinted in the quantity of food given them while being themselves artificially infected with the tubercle bacillus. Experiment No. 2. Five healthy rabbits were placed under the following conditions: A fresh hole about ten feet deep was dug in the middle of a field, and the animals having been confined in a small box with high sides but no top, were lowered to the bottom of…
33. Which statements concerning passive and active transport are correct? A. Both passive and active transport requires cell energy B. Passive requires cell energy while active transport does not C. Active transport requires cell energy while passive does not D. Neither passive nor active transport requires cell energy
Effect of hypo, hyper and isotonic solutions in animal and plant cells and including the terms plasmolysis, hemolysis, turgor pressure
Osmosis is often defined as the flow of water through a semipermeable membrane, from a dilute solution to a more concentrated solution. Video experiment #1 – saturated sucrose/dialysis tubing/water osmosis from U Mich. https://youtu.be/pCupvFGN4bw A saturated solution of sucrose-containing a red dye is separated from water by dialysis material. What happens to the volume of sucrose solution during the experiment? How do you know that the sucrose solution did not flow into the beaker of water? (Give evidence from your observation of the experiment.) Diffusion, Osmosis, Dialysis video: https://youtu.be/tHzkRtzVmUMDialysis, the flow of both solvent and small molecules or ions through a dialyzing membrane, is important clinically in the operation of the artificial kidney machine.
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