Explain briefly the differences in Rf values of the pigments that you have separated from spinach juice. What properties must the pigment have to travel further in the chromatogram? Consider the structures of cholesterol and ?-carotene. Which do you think will have the highest Rf value if the solvent used was acetone - hexane (20:80)? Why?

Explain briefly the differences in Rf values of the pigments that you have separated from spinach juice. What properties must the pigment have to travel further in the chromatogram? Consider the structures of cholesterol and ?-carotene. Which do you think will have the highest Rf value if the solvent used was acetone - hexane (20:80)? Why?

Macroscale and Microscale Organic Experiments

7th Edition

ISBN:9781305577190

Author:Kenneth L. Williamson, Katherine M. Masters

Publisher:Kenneth L. Williamson, Katherine M. Masters

Chapter47: Martius Yellow

Section: Chapter Questions

Problem 2Q

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Similar questions

Explain briefly the differences in Rf values of the pigments that you have separated from spinach juice. What properties must the pigment have to travel further in the chromatogram?
Consider the structures of cholesterol and ?-carotene. Which do you think will have the highest Rf value if the solvent used was acetone - hexane (20:80)? Why?
Solvent #1 is a 50:50 mixture of hexane and ethyl acetate and solvent #2 is 25% hexane. Which solvent mixture is more polar: the 50:50 mixture or the 25:75 mixture? Explain briefly What would happen to the Rf of the spots in solvent #2 compared to solvent #1? Why?
A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most attracted in the stationery phase and why?
If the TLC eluent increases in polarity, what will happen to the Rf value of acetaminophen and phenacetin? Explain.
A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most soluble in the mobile phase and why?
For Fluorenonce and benzoic acid 1. Which compound would you expect to be the top spot on the TLC and why? 2. Which compound would you expect to be the first to come off the column and why? 3. What data supports the identification of the compound in a yellow fraction?
What would happen to the Rf values of the pigments if you were to increase the relative concentration of acetone in the developing solvent?
A student has a green dye mixture on which she performs a TLC experiment with a silica gel plate and acetone as the solvent. She obtains a yellow spot with an Rf of 0.75 and a teal spot with an Rf of 0.45. Explain the significance of her data.
A mixture composed of biphenyl, benzoic acid, and benzyl alcohol is spotted on a silica gel TLC plate and eluted with a 10:1 hexanes:ethyl acetate solvent mixture. In what order are the Rf values of the 3 compounds (lowest first)?
Why does a 70:30 hexane:acetone mixture produce a more effective separation of pigments in spinach compared to a 50:50 hexane:acetone mixture? Explain thoroughly
Which of the following carotenoid structures below would you expect to have the lowest RF in a chromatography experiment using an ether-petroleum ether mobile phase? Why? Lycopene: β-carotene: Vitamin A