Which of the following carotenoid structures below would you expect to have the lowest RF in a chromatography experiment using an ether-petroleum ether mobile phase? Why? Lycopene: β-carotene: Vitamin A
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- Which of the following carotenoid structures below would you expect to have the lowest RF in a chromatography experiment using an ether-petroleum ether mobile phase? Why?
Lycopene:
β-carotene:
Vitamin A
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- The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67A thin layer chromatography experiment is planned to determine the Rf values of four compounds. List the following compounds in order of increasing Rf values from shortest to longest. (If B is shortest, then A, C, and D is longest, enter BACD )Which two of compounds below will be difficult to be separated by column chromatography (using ethanol as solvent) and why? -p-anisic acid -methyl-4-aminobenzoate -benzoic acid -ethyl-3-hydroxybenzoate -ethyl-4-nitrobenzoate -trans-cinnamic acid -benzanilide -2-methyl-1-nitronapthalene -9-fluorenone -naphthalene -trans-stilbene -vanillin
- Explain briefly the differences in Rf values of the pigments that you have separated from spinach juice. What properties must the pigment have to travel further in the chromatogram? Consider the structures of cholesterol and ?-carotene. Which do you think will have the highest Rf value if the solvent used was acetone - hexane (20:80)? Why?A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most soluble in the mobile phase and why?Would you expect an alcohol or an ether to have a higher Rf value on silica gel thin layer chromatography? Why?
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- Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most attracted in the stationery phase and why?Greener synthesis of Bupropion Hydrochloride What spot on TLC would be higher for an eluent system of 1:1 acetone: hexane, when bupropion hydrochloride is spotted against m-chloropropiophenone?