Explain how figure 3 illustrates that the C-terminus of the EcoSSB is responsible for binding to the X subunit.
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- Which RNA conformation favors translation—the form with the Shine-Dalgarno antisequestor or the form in which the Shine-Dalgarno sequence is within a stem-loop?Describe the sequence in bacterial mRNA that promotes recognition by the 30S subunit.Figure 28.41 gives some examples of recombination in IgG codons 95 and 96, as specified by the Vkand Jkgenes. List the codon possibilities and the amino acids encoded if recombination occurred in codon 97. Which of these possibilities is less desirable?
- . a. How many ribosomes are required (at a minimum)for the translation of trpE and trpC from a singletranscript of the trp operon?b. How would you expect deletion of the two tryptophan codons in the RNA leader to affect theexpression of the trpE and trpC genes?Explain three types of post-transcriptional modifications to RNA.A shortened version (apo B-48) of apolipoprotein B is formed by the intestine, whereas the fulllength protein (apo B-100) is synthesized by the liver. A glutamine codon (CAA) is changed into a stop codon. Propose a simple mechanism for this change.
- Describe the recognition process by whichthe tRNA for N-formylmethionine interacts with the portion ofmRNA that specifies the start of transcription.Give a brief description of how RNAi works in cells specifying the molecules involved?How does the cell know that a polypeptide needs to be translated at the ER? This is a particular feature of the polypeptide.
- Using three examples, describe how allosteric sites are important in the function of genetic regulatory proteins.Explain how an α helix in a transcription factor protein is able to function as a recognition helix.Explain why knowing the orientation of a promoter is critical when determining the amino acid sequence of an encoded protein.