Explain how the color change occurs with the biuret reagent in the presence of proteins
Q: Why is hydrolysis of sucrose also known as specific sucrose test?
A: Hydrolysis is a chemical reaction where a molecule of water ruptures one or more chemical bonds. The…
Q: Kovac's reagent is added to detect indole. True or False?
A: True
Q: What is a folin-ciocalteau reagent and how can it be used to determine the concentration of uric…
A: Uric acid is the end product formed by the catabolism of purine bases. The normal range of uric acid…
Q: Give two advantages to using the biuret reaction to measure protein concentration compared to…
A: A Biuret test is a general test that is given by compounds containing two or more peptide bonds…
Q: The lipopolysaccharide of Gram-negative bacteria is composed of the following EXCEPT:
A: Bacteria are characterized by the presence of a cell wall but not all bacteria have cell walls.…
Q: A protein 'standard curve' is a graph showing how absorbance changes with the amount of protein True…
A: Proteins are biomolecules which is made up of aminoacids connected via peptide bonds. These are of…
Q: Many proteins are secreted in their inactive form. This is also true of many toxic proteins produced…
A: Toxic proteins are produced by microbes for infecting their host, prevent attack by other microbes,…
Q: What is the mechanism of the reaction of the Bradford reagent with proteins? (explain in not less…
A: The Bradford protein assay is used to measure the concentration of total protein in a sample.
Q: Why is Molisch’s test used for the determination of presence of pentose in the hydrolysate? What…
A: Carbohydrates are polyhydroxy compounds, composed of carbons, hydrogen, and an oxygen atom. It is…
Q: Show the complete condensation reaction to form maltose. Label all molecules.
A: Condensation reaction to form maltose A disaccharide is produced by joining 2 monosaccharide (single…
Q: what is the purpose of adding HCl in the precipitation of proteins by alkaloidal reagents?
A: Protein precipitation is the process in which protein is separated from any extra contaminants that…
Q: In detection of urea in urine what are the mechanisms, reactions, possible result and interpretation…
A: Urea is considered to be a waste product synthesized in the body by the oxidation of amino acids…
Q: Which test can show the hydrolytic reaction of a protein by Trypsin?
A: Amino acids are organic molecules involved in the condensation reaction to form long protein chains.…
Q: Explain the following given terms: (a) Phenylketonuria, (b) Thalassemia.
A: Mutation: Normal DNA contains a particular sequence of DNA. If the sequence of DNA is changed due to…
Q: Study the given structures below. Which of the following are epimers? H 0 H- OH но H- H- HO- но -H…
A: Epimers are the stereoisomers that differ in arrangement of groups in a single chiral carbon. A…
Q: Penicillin reacts with the cysteine residue of penicillin binding protein. Show the reaction here.
A: Penicillin is a beta-lactam antibiotic. Penicillin kills the bacteria by inhibiting the synthesis of…
Q: Lead acetate solution is an agents of denaturation. Explain in 10 sentences the effect of each on…
A: Denaturation is the process in which the protein loses its structural configuration and as a result,…
Q: The six tests for the detection of functional groups in amino acids and proteins. TEST REAGENTS…
A: test REAGENTS POSITIVE RESULT POSITIVE INDICATION NINHYDRIN NINHYDRIN(indane-1,2,3-trione hydrate)…
Q: otal protein is used.
A: Proteins can be defined as large biomolecules that consist of one or more long chains of amino acid…
Q: which ane of the following reagents cannot be used for determination of lipid concentrations?…
A: Lipid is a molecule which is a part of cell and cell membrane. It gets dissolved only in nonpolar…
Q: How much NS was diluted? b. How much Gentamicin was diluted? c what is the desired concentration?
A: Question is related to drug calculation. Available gentamicin dose is - 20 mg/ 2 ml Desired dose is…
Q: Why absorbance is taken at 550nm in Biuret test? Does this absorbance change based on the detection…
A: In the UV-visible area of the spectrum, biological macromolecules including proteins and nucleic…
Q: precipitation of proteins is done by all of these except -
A: Proteins are made up of amino acids joined together by peptide bonds. Proteins can be precipitated…
Q: Do all the amino acids with aromatic side chains give positive result to Xanthoproteic acid test.…
A: Amino acids are biologically important as they are the primary building blocks of proteins. The…
Q: Briefly explain how the protein test for dairy products was cheated by the adulteration of the…
A: Adulteration denotes that the food product does not meet the required food safety and legal…
Q: What will happen to the color of the Coomassie reagent as more protein is added?
A: The Pierce Coomassie also known as Bradford is a Protein Assay Kit. Is changes the colour of…
Q: In the precipitation of Proteins by heavy metal salts, are the salts completely removed from the…
A: Adding chaotropic substances like the salts, increases the entropy and can thus destabilize the…
Q: Instead of Molisch's test, what specific test can be used to detect the presence of pentose sugar?
A: A monosaccharide that has five carbon atoms is referred to as pentose sugar. Ribose present in RNA…
Q: How smith's test gives a green ring result in urine as a presence of bile salts? What is the…
A: Smith's test is to check the bile pigment.
Q: Explain why protein purification procedures: normally carried out 4-5°C. are
A: Crude extracts that contain the protein of interest should be refrigerated, and the recombinant…
Q: Describe the effect of the amino acid change on protein function: how does the HbS protein function…
A: Proteins are the polymers of amino acids. These amino acids are linked together by peptide bond. Now…
Q: Explain the observed results of unboiled starch –saliva mixture with iodine solution and fehling's…
A: Starch or amylum is a polymeric sugar comprising of various glucose units joined by glycosidic…
Q: Provide examples of amino acids that would yield a positive Azo Dye test and amino acids that would…
A: The azo dye test is used to identify the presence of amino acids that contain the aromatic ring.…
Q: Consider the decapeptide angiotensin I. Treatment of angiotensin I with ACE (the…
A: Angiotensin : It is a peptide hormone that causes vasoconstriction and an increase in blood…
Q: Are Xanthoproteic and MillonNasse tests satisfactory for use in the urinary examination for protein?…
A: The pH of urine varies from 5.5 to 7.5. If the diet is rich in proteins, sulfuric and phosphoric…
Q: Discuss the functions and components of Biuret dye in protein content determination.
A: Since you have asked multiple questions, we will answer only first question for you. In order to get…
Q: Would the ninhydrin test useful and reliable for showing the presence or absence of proteins?
A: Proteins are the most extensive class of biological macromolecules in terms of chemical and…
Q: Is it true that coconut oils contain no cholesterol? Provide reasons
A: Coconut oil is basically a tropical oil derived from the dried nuts of coconut palm tree.
Q: In what way can alkaloidal reagents precipitate proteins?
A: Proteins exist in colloidal solution due to hydration of polar groups (-COO-, -NH3+, -OH). Proteins…
Q: If I had a standard with 2.0 mL of Bradford reagent then added 50μL of 250 μL/mL ; what would be the…
A: The Bradford protein assay is commonly used due to its sensitivity, speed, convenience, lack of a…
Q: Why would you expect to see no violet color when free amino acids (no peptide bonds) are mixed with…
A: Biuret test is used to detect the presence of peptide bonds in a sample. The Biuret reagent is…
Q: Describe Ninhydrin test for proteins with the reaction
A: Qualitative tests help to detect the presence of substances by giving visible reactions such as…
Q: Differentiate sweet molecules in Column A using the criteria in column B.
A: Acesulfame - K:- It is an artificial sweetener that is calorie-free, mostly used in sugar-free…
Q: Is there a possibility for lactose to give a positive Mucic Acid test? Explain.
A: Monosaccharides upon treating with oxidizing agents here, nitric acid yield dicarboxylic acids by…
Q: How to explain that milk protein casein curdles (precipitates) during boiling if the milk is sour?
A: Casein is precipitated by simply adjusting the pH of the milk to be sufficiently acidic that the…
Q: how do salt bridges that include amino-terminal carbamate stabilize the deoxy form of hemoglobin.…
A: Hemoglobin is a protein which transports oxygen and carbon dioxide to the tissues and to the lungs…
Q: components of robert's reagent
A: Robert's reagent is type of chemical reagent which is used for determining the quality of albumin…
Q: One test for the presence of many simple carbohydrates is to use Benedict's reagent. What is the use…
A: Benedict's test is used to test for simple carbohydrates. The Benedict's test identifies reducing…
Explain how the color change occurs with the biuret reagent in the presence of proteins
Trending now
This is a popular solution!
Step by step
Solved in 2 steps