Why absorbance is taken at 550nm in Biuret test? Does this absorbance change based on the detection test or specific amino acid being tested?
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Why absorbance is taken at 550nm in Biuret test? Does this
absorbance change based on the detection test or specific amino
acid being tested?
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- Rx Of oxacinophtalmic solution 3% Disp. 10 mL How many milligrams of axacin contained in each milliter of the dispensed prescription?How do HABA, salicylate, and acetylsalicylic acid bind to BSA? Why do the absorbances for each of the HABA amounts have to measured with and without BSA? will give ratingWhen comparing the biuret reaction to measuring protein absorbance directly at 280 nm, list two benefits of utilizing the biuret reaction to quantify protein concentration.
- The absorbance values for different BSA standards. How can the protein concentration be calculated? Which absorbance value should be used?Calculate the concentration of 0.2ml amino acid when path length is 1cm and has an absorbance value of 0.50, if a 0.5ml solution of same amino acid has a concentration of 0.72mg/ml with an absorbance of 0.9.If a protein lysate obtained is 1003 ug/ml What would be the total concentration of each lysate that is loaded into their well if you load just 25 ul of each?
- What is the [CH3COO−]/[CH3COOH] ratio inan acetate buffer at pH 4.00?Why is the absorbance spectrum of the iron (II) complex obtained first prior to measuring the absorbance of the standard concentrations and unknown samples?How smith's test gives a green ring result in urine as a presence of bile salts? What is the mechanism, reaction, possible results and interpretation of bile salts wirh smith's reagent?
- give two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm.Give two advantages and two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nmGive two benefits of utilizing the biuret reaction to assess protein concentration over directly measuring protein absorbance at 280 nm.