Explain one by one why the results is like that for 4 test tubes in pancreatin digestion specifically for protein digestion B. Pancreatin digestion1. Prepare 4 test tubes as follows:Tube 1 - 3 ml neutral pancreatinTube 2 - 3 ml waterTube 3 - 3 ml water + 1.5 ml pancreatinTube 4- 3 ml water + 1.5 ml pancreatin + 1.5 ml 1% Na2CO32. Place equal size of hard-boiled egg white in all tubes.3. Place all tubes in water bath with a temperature maintained at 40°C_for 1.5hours.4. Add 3 drops of toluene in all tubes and incubate till the next lab period.5. Observe the size of the egg white in each of the tubes.6. Take 1 ml of the supernatant in each tube and place in 4 separate test tubeslabelled 1b,2b,3b,4b. Add 1 ml of 10% NaOH and 2 drops of 1% CUSO4 in eachtube. Mix and compare the result of the 4 tubes. B. Pancreatin DigestionTUBESIZE of egg whiteResultsPurple colored Solution1(Pancreatin)No change in sizeNo change in sizePurple colored Solution2(Water)No change in sizeLight-Purple colored Solution3(water, Pancreatin)(Ctrl) -Blue colored solutionSize decreased4(water, Pancreatin,Na2CO3)greatly

Pancreatin is a medication which is mixture of various digestive enzymes. These enzymes are…

Answered: Explain one by one why the results is…

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter4: The Metric System

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Procedure: A. Peptic digestion 1. Prepare 4 test tubes as follows: Tube 1 - 3 ml pepsin Tube 2 - 3 ml of 0.4% HCl Tube 3 - 3 ml pepsin + 1.5 ml of 0.4% HCl Tube 4 - 3 ml pepsin + 1.5 ml of 1% Na2CO3 2. Add equal slices of hard-boiled egg white to each tube. 3. Cover and incubate at 40oC for 2 hours. 4. Add 4 drops of toluene and store till the next lab period. 5. Determine the extent of digestion by visually noting the size of the hard-boiled egg white in each tube. 6. Withdraw 1 ml of the supernatant liquid from each tube and place in 4 separate test tubes labelled tube 1a. 2a, 3a, 4a. 7. Neutralize tubes 2a& 3a with solid sodium carbonate. 8. To each supernatant liquid, add 1 ml of 10% NaOH and 2 drops of 1% CuSO4.Compare the colors obtained in the 4 tubes.9. Place 1 ml of 0.5% peptone in a test tube and add 1 ml of 10% NaOH and 2drops of 1% CuSO4. Compare the result of these tubes with the other 4 tubesabove.B. Pancreatin digestion1. Prepare 4 test tubes as follows:Tube 1 - 3…
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