n which tube is there evidence of hydrolysis of protein in peptic and pancreatin digestion
n which tube is there evidence of hydrolysis of protein in peptic and pancreatin digestion
Chapter19: Child Health
Section: Chapter Questions
Problem F1CRE
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- In which tube is there evidence of hydrolysis of protein in peptic and pancreatin digestion?
Transcribed Image Text:Procedure:
A. Peptic digestion
1. Prepare 4 test tubes as follows:
Tube 1 - 3 ml pepsin
Tube 2 - 3 ml of 0.4% HC1
Tube 3 - 3 ml pepsin +| 1.5 ml of 0.4% HCl
Tube 4 - 3 ml pepsin + 1.5 ml of 1% NazCO3
2. Add equal slices of hard-boiled egg white to each tube.
3. Cover and incubate at 40°C for 2 hours.
4. Add 4 drops of toluene and store till the next lab period.
5. Determine the extent of digestion by visually noting the size of the hard-boiled egg
white in each tube.
6. Withdraw 1 ml of the supernatant liquid from each tube and place in 4 separate test
tubes labelled tube la. 2a, 3a, 4a.
7. Neutralize tubes 2a& 3a with solid sodium carbonate.
8. To each supernatant liquid, add 1 ml of 10% NaOH and 2 drops of 1% CuSO. Compare
the colors obtained in the 4 tubes.
9. Place 1 ml of 0.5% peptone in a test tube and add 1 ml of 10% NaOH and 2 drops of
1% CuSO4. Compare the result of these tubes with the other 4 tubes above.
B. Pancreatin digestion
1. Prepare 4 test tubes as follows:
Tube 1 - 3 ml neutral pancreatin
Tube 2 - 3 ml water
Tube 3 - 3 ml water +1.5 ml pancreatin
Tube 4 - 3 ml water + 1.5 ml pancreatin + 1.5 ml 1% NaCO;
2. Place equal size of hard-boiled egg white in all tubes.
3. Place all tubes in water bath with a temperature maintained at 40°C for 1.5 hours.
4. Add 3 drops of toluene in all tubes and incubate till the next lab period.
5. Observe the size of the egg white in each of the tubes.
6. Take 1 ml of the supernatant in each tube and place in 4 separate test tubes labelled
1b,2b,3b,4b. Add 1 ml of 10% NaOH and 2 drops of 1% CuSO4 in each tube. Mix and
compare the result of the 4 tubes.
Transcribed Image Text:RESULTS:
A. Peptic Digestion
Biuret test result
SIZE of egg white
no change in size
TUBE
violet solution
1 (реpsin)
light violet solution
blue solution
violet solution
2 (HCI)
minimal change
became smaller
no change in size
3(pepsin, HCI)
4(pepsin, Na:CO:)
5 PEPΤONE
dark violet solution
B. Pancreatin Digestion
TUBE
1(Pancreatin)
2(Water)
3(water, Pancreatin)
4(water, Pancreatin,
Na:CO;)
SIZE of egg white
no change in size
no change in size
no change in size
Biuret test result
violet solution
violet solution
violet solution
became smaller
blue solution
1. In which tube is there evidence of hydrolysis of protein in peptic and pancreatin
digestion?
Peptic
Pancreatin
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