Explain the concept of and apply the aseptic technique
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Explain the concept of and apply the aseptic technique
Explain hanging drop and wet mount preparations of microbial specimens
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- Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.Outline the different physical and chemical methods of sterilization. Briefly describe how each method control microbial growthexplain in your own words an introduction to a lab about Control of Microbial Growth - Antimicrobic Sensitivity Testing Kirby-Bauer Method
- Describe the five phases of a microbial growth curve observed when microbes are grown in a batch cultureIndicate the correct way of inoculating the following media. Plate Media For sensitivity testing ________________________________________________ For isolation of colonies ___________________________________________________Explain the mechanism of action of several of the physical and chemical agents employed to control microbial growth.
- Propose a confirmatory procedure that will determine if the microbial growth recovered from canned food that displays no evidence of spoilage is from the canned product itself or from laboratory contaminationExplain the significance of aseptic culture technique in growing microorganisms.Defi ne radiation and assess the use of irradiation as a method of microbial control.
- Describe the microbial growth on the plates overlaid with water sample (tap water).List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?distinguish between antiseptics and disinfectants. Are the terms interchangeable? explain quantitative and qualitative proceedure in effects of chemicals in microbial populations.