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- What are the parameters that can be used for analytes identification and quantification using Cyclic Voltammetry, respectively?What are the common components of the buffers used in hydrophobic column chromatography? Explain the purpose of each component.Discuss the advantages and disadvantages of sequential versus simultaneous multichannel ICP spectrometers.
- Discuss the advantages, disadvantages, and limitations of the technique of cyclic voltammetry in both qualitative and quantitative measurements.What are the applications of utilizing molecular/size exclusion chromatography in the analysis of samples?IN GC, What effect does changing from a temperature ramp to an isothermal temperature program have on resolution of peaks at the beginning of the chromatogram versus the end of the chromatogram. Consider molecular weight and boiling point of the analytes.
- In color chromatography of plant pigments, what complications would a dried-out column (solvent level is below the top of the silica) introduce to the elution and isolation of pigments?In your own words answer the following With reference to the Van Deemter equation, explain thoroughly whether separation may be expected to improve in an Liquid solid chromatography LSC column with a 5 μm particle size or the one with a 20 μm particle sizeTwo species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are separated by elution on silica gel with hexane as eluent. The ratio for the packing VS/VM =0.425 Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are separated by elution on silica gel with hexane as eluent. The ratio for the packing VS/VM =0.425a. Calculate the retention factor for each soluteb. Calculate the selectivity factorc. How many plates are needed to provide a resolution of 1.5?d. What column length should be used to provide a resolution of 1.5 if the plate height ofthe packing is 1.5 ×10-3 cm?e. If the flow rate is 6.75 cm min-1, how long will it take to elute the two species?
- Explain how to obtain larger particles based on the relative supersaturationWhich of the following is TRUE regarding instrumental methods of analysis? A. these methods have lower throughput compared to classical method. B. these methods have lower accuracy compared to classical methods. C. these methods can measure lower analyte concentration than classical methods D. these methods have higher precision compared to classical methods.In reversed phase HPLC, how will the retention time of a component of a mixture vary with the polarity of its molecules? Explain your answer