Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are separated by elution on silica gel with hexane as eluent. The ratio for the packing VS/VM =0.425 Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are separated by elution on silica gel with hexane as eluent. The ratio for the packing VS/VM =0.425 a. Calculate the retention factor for each solute b. Calculate the selectivity factor c. How many plates are needed to provide a resolution of 1.5?
Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are separated by elution on silica gel with hexane as eluent. The ratio for the packing VS/VM =0.425
Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16. They are separated by elution on silica gel with hexane as eluent. The ratio for the packing VS/VM =0.425
a. Calculate the retention factor for each solute
b. Calculate the selectivity factor
c. How many plates are needed to provide a resolution of 1.5?
d. What column length should be used to provide a resolution of 1.5 if the plate height of
the packing is 1.5 ×10-3 cm?
e. If the flow rate is 6.75 cm min
-1
, how long will it take to elute the two species?
Trending now
This is a popular solution!
Step by step
Solved in 4 steps with 3 images
