Explain why it is essential that bacteria cultures be sterilized in an autoclave.

Q: Describe how an appropriate inoculum can be usedto successfully start an enrichment culture.

A: Enrichment culture is culturing a specific microorganism using certain growth media that favors…

Q: What advantages do solid media offer for the isolation ofmicroorganisms?

A: Micro-organism are those that cannot be seen by the naked eye, but are visible under microscopes.…

Q: Why does a precipitate form on Sierra’s medium when inoculated with a lipolytic organism? Discuss…

A: Nutrient mediums are used to provide growth to the microorganism in invitro experiments. These…

Q: Define agar.

A: A dried jelly-like substance called agar, commonly referred to as agar-agar, is made from the red…

Q: Why is dilution a necessary part of pure culture preparation?

A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…

Q: Why are biochemical tests used to identify microbes?

A: Microbes are living organisms that are very small and can be seen only with the help of a…

Q: Why is CFU more applicable to a culture of streptococcus than to a culture of E.coli

A: Streptococcus is a Gram-positive bacteria that are spherical in shape. This bacteria is responsible…

Q: Why is it important to keep the culture at the correct incubation temperature when measuring the…

A: Generation time is the time which is taken by the bacteria to double in number during a specified…

Q: Why is it important for a microbiology laboratory technician to use aseptic technique?

A: The majority of the microbes onto which scientists work in laboratories are known pathogens.

Q: Why is it necessary to maintain aseptic conditions in microbiology?

A: Aseptic conditions means microbs free environment. Microbs means bacteria, virus and fungi. It may…

Q: Define sterilization.

A: Microbial control indicates the prevention or inhibition of microbial growth. It involves physical…

Q: discuss the significance of the different aseptic techniques utilized in maintaining sterility of…

A: The laboratory that carries out growth of prokaryotic cells or eukaryotic cells in physiological…

Q: what type of bacteria would be used to make sauerkraut?

A: Sauerkraut is product of cutting raw cabbage that fermented by various lactic acid bacteria. The…

Q: micro-organisms are the most resistant to heat sterilization?

A: Most of the bacteria cannot survive adverse heat condition. But somehow particular bacteria can…

Q: Which of the following statements is true regarding steam sterilization? a. It is a universal…

A: steam sterilization , also called autoclave sterilization , is used in microbiological laboratory in…

Q: Under what conditions are media typically sterilized in an autoclave?

A: An autoclave is the pressure chamber used to carry out the scientific and industrial processes…

Q: CTSA growth medium designed

A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…

Q: Explain why sulfa drugs prevent bacterial growth without harming the human host.

A: Antimicrobial drugs are those which destroy or inhibit the growth of microorganism. They have their…

Q: Why are semisolid media sometimes preferred over slide techniques for evaluating bacterial motility?

A: Semi-solid media are soft media that is used in demonstrating the motility of bacteria and…

Q: Why should culture media after inoculation be incubated at an optimal temperature immediately?

A: Culture media, also called as growth media, are particular combinations of nutrients and other…

Q: What advantages do solid media offer for the isolation of microorganisms?

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: Why is PBS buffer preferred when preparing a monolayer cell culture?

A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…

Q: If you had a mixed culture that contained a Gram-negative enteric bacterium and Gram-positive…

A: MSA or mannitol sale agar used in microbiology. It encourages growth of some group of bacteria…

Q: Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation?

A: Introduction Microbial culture is a microbiological technique that involves actively multiplying…

Q: Explain why it is important to use only a small amount of bacteria when preparing a smear

A:

Q: What are some advantages and disadvantages of using ionizing radiation to sterilize food?

A: Sterilization involves physical methods for removing or controlling the microbes. The physical…

Q: What settings and time are normally used for sterilization in an autoclave?

A: The fundamental rule of steam sterilisation as cultivated in an autoclave, is to open every thing to…

Q: which method do you think is more reilable, a test utilizing microbial growth media, or a…

A: The investigation of microorganisms is significantly aided by the ability to culture them, that is,…

Q: Heat-labile material to be introduced to culture medium was filtered using membrane filters with…

A: given that the heat labile material which is introduced to culture media is filtered using a…

Q: What makes agar good support for microbial growth?

A: Microbial culture is a method of multiplying microbial organisms by letting them reproduce in…

Q: Why must water be subjected to microbiological analysis?

A: Water is used for various purposes in daily lifestyle including food. Thus, it should be monitored…

Q: Distinguish heat sterilization and "commercial sterility".

A: lowering the prevalence of harmful bacteria until they are no longer a health threat. usually…

Q: Why is 15 minutes generally used for sterilization? Why is agar added to medium?

A: Sterilization is the process in which all life forms like microbes (fungi, bacteria, viruses,…

Q: How can moist heat sterilization can sterilize antibiotic solution?

A: Moist heat sterilization is a technique that uses heated, increased pressure steam to sterilize an…

Q: What is the most difficult type of microbial cell or structure to kill? What methods can be used to…

A:

Q: Discuss how can some anaerobes be maintained in pure cultures?

A: Introduction :- A pure (or axenic) culture is a population of cells or multicellular creatures that…

Q: How can organisms develop resistance to chemical disinfectants?

A: Bacteria can develop resistance to disinfectants over time either by acquisition of exogenous mobile…

Q: What products are sterilized using high energy ionizing radiation?

A: Sterilization is the process of killing microorganisms.

Q: Why do we grow the bacteria in minimal media when testing for citrate utilization

A: Minimal media are those that provide the minimal nutrients possible for colony development, usually…

Q: Why are plated media stored in an inverted position?

A: Plated media enables the isolation of microorganisms from samples or specimens.

Q: Write the limitations of sterilization by Dry heat?

A: The dry heat sterilization technique includes the removal or killing the microorganism and require…

Q: What method can be used to achieve sterilization?

A: It is required to describe the method to be used to achieve sterilization.

Q: Why is chlorination, although beneficial in terms of bacterial pathogen control, of environmental…

A: Chlorination is a disinfection procedure in which microorganisms are killed by the use of chlorine…

Q: Suppose your Professor handed you a test tube with 2.0mL of E.coli broth culture and told you to…

A: In both basic and applied research, "microbiology" is a highly significant area. Proteomics,…