Explain why it is important to wash the wells with PBS between the additions of each reagent?

Q: Why Do not add more than 2 drops of copper sulfate in Biuret reagent?

A: There are several biochemical tests to identify the presence of protein in a sample. There are tests…

Q: Between Enterobacter aerogenes and Enterobacter cloacae, which are positive or negative for methyl…

A: methyl red and Voges Proskauer tests both are biochemical tests for identifying bacteria in…

Q: Explain the purpose of thiosulfate in the medium.

A: Culture media is used to enrich the number of bacterial growth, to select certain bacteria and…

Q: What are the common elements of puncture wounds, crush injuries,and compound fractures that…

A: Clostridium perfringens and a few other species of Clostridium cause necrotic tissue infections in…

Q: Define sterilant, and name the principal sporicidal chemical agents.

A: Sterilization is defined as the process where all the living microorganisms, including bacterial…

Q: Explain the Alkali reserve ?

A: Alkali reserve is represented by Plasma bicarbonate . Bicarbonate concentration is significantly…

Q: What is the logic behind testing for E. coli to detect fecalcontamination of water?

A: Bacteria are unicellular prokaryotic organism that maintains a definite shape since it contains the…

Q: What is the media used in Catalase test and its expected results?

A: Catalase is an enzyme that is found in the cell in most of the organism that protect the cell from…

Q: Short description of the process occurring at each step of the extraction in the miniprep process…

A: A bacterium's plasmid is a circular, double-stranded extrachromosomal DNA with two strands. It's…

Q: explain why disinfectants that has a residual is preferable to one that does not

A: Introduction Disinfectants are chemical substances or compounds that are used to kill or inactivate…

Q: Explain why litmus milk is considered a good differential medium.

A: Differential media are the growth medium that helps in differentiating bacterial samples on the…

Q: In using the centrifuge machine, how will this be remedied if there are only 3 separate liquid…

A: Centrifuges are laboratory equipment that is used to separate components in a mixture based on their…

Q: Why does the nitrate reduction tube turn red after the addition of zinc?

A: Nitrate reduction test is used to find the bacteria who have the ability to reduce the nitrate.

Q: Explain how the type of hemolysis can be used to tell apart two species that would look the same…

A: Hemo means blood and Lysis means destruction so hemolysis is destruction of blood. Bacteria show…

Q: For which diseases can one not rely upon chlorination of water as a method of control? Explain why…

A: Thyroid disease has been practically eliminated by using the chlorination of water. The treatment of…

Q: Which would be more likely to cause illness and why—a water sample that tested positive for fecal…

A: Ans: Escherichia coli  is the gram negative organism which forms part of normal flora of humans.

Q: How does the concentration of H+ change when a culturemedium at pH 5 is adjusted to pH 9?

A: pH is the measure of acidity or basicity of a solution. If pH of a solution is 7, then it is…

Q: What is demanufacturing, and why is it used for some types of waste?

A: Demanufacturing was suggested as a way to reduce the environmental footprint while maintaining the…

Q: In the multiple fermentation tube technique does the absence of gas formation after 24 hours…

A: Coliform microbes are life forms that are available in the climate and in the defecation of all…

Q: why do microorganisms differ in their response to disinfectants

A: Microorganisms are the minute microscopic organism that is generally invisible to the human eye.…

Q: You have been assigned to your first comatose patient, and you notice 0.12% chlorhexidine gluconate…

A: Chlorhexidine gluconate solution: Chlorhexidine gluconate is used as oral rinse to prevent…

Q: Explain why sulfa drugs prevent bacterial growth without harming the human host.

A: Antimicrobial drugs are those which destroy or inhibit the growth of microorganism. They have their…

Q: Explain why the following step are necessary during subculturing:  Flaming the neck of…

A: Aseptic technique-Aseptic technique should be observed whenever microorganisms are transferred from…

Q: In the differential absorption heterophile test, what findings are diagnostic of IM?

A: Infectious mononucleosis is a very common disease in adults with symptoms such as malaise,…

Q: Which microorganism does not contain superoxide dismutase enzymes?

A: Superoxide dismutase (SOD) is an enzyme found altogether in living cells. An enzyme could be a…

Q: Does a positive presumptive test mean that the water is absolutely unsafe to drink? Explain

A: Water is tasteless, transparent, inorganic. Colorless, and odorless chemical substance. Its chemical…

Q: Is colistin Naladixic acid agar selective and/or differential? Explain

A: C-CNA (Columbia-CNA Agar) is a form of selective media that favours Gram-positive bacteria.

Q: Explain why it is important to use only a small amount of bacteria when preparing a smear

A:

Q: What is the media used in Urease Test and expected results?

A: Urease test is defined as a biochemical test that distinguishes the basic alkaline fermentation of…

Q: Using aseptic techniques, list the general procedure of inoculating a broth from a plate culture.

A: Aspetic techniques that you should follow during inoculating a broth from a plate culture are:…

Q: Explain why the following steps are necessary during subculturing:  Cooling the inoculating loop…

A: Subculture is the process of the removal of the media and transferring the cells from previous…

Q: For what the stirred tank bioreactors have been designed?

A: Bioreactors are generally used for microbial fermentation processes. It has a headspace volume and…

Q: What is the connection of the effects of Acidification and chemical preservatives on Microbial…

A: OFSP means Orange - fleshed sweet potato. It is a special type of sweet potato with high levels of…

Q: Composition (+) of reagents Test Importance of the test Group Result Responsible

A: Proteins are macromolecules formed by amino acids; they are essentially polymers of structural units…

Q: Which of the tubes (containing thigylcolate broth) best illustrates the growth of facultative…

A: Growing bacteria in thioglycolate tube cultures allows us to easily observe different molecular…

Q: in the isolation of alkaloid,in the early stages of acid or base required conditions. explain the…

A: Alkaloids are secondary metabolites derived through shikimic acid pathway. They are nitrogen…

Q: why should the ethanol/lystate boundary not be distrubed

A: Ethanol lysate boundary is very much important in the case of DNA extraction experiments, where…

Q: Explain the rational of using saponification-solvent extraction to extract pyrene from milk

A: Asked : Rational of using saponification-solvent extraction to extract pyrene from milk

Q: Which among the ingredients of this product is the main preservative? Explain.

A: For centuries, people have been preserving food. It all began with the ancient practice of…

Q: What kinds of organisms may be plentiful in a milk sample, yet give a negative reductase test?

A: Methylene blue reduction test is based on the principle of oxygen reduction by increased microbial…

Q: Other than the PYR test, what is another use of the PEP reagent?

A: PYR is a colorimetric method used to detect the presence of pyrolidonyl arylamidase activity in a…

Q: Illustrate the dilution series used and label the final dilution of each dilution.

A: The serial dilution method is regularly used in the microbiology lab. In environmental samples like…

Q: explain methods for cleaning beans using flat bed screen and drum spray cleaner

A: Much of the mechanical cleaning procedures must be performed in the field for proper storage of the…

Q: List the ingredients and recipe (in gram or milligram) to make one liter (1000 ml) of Trypticase Soy…

A: A growth medium can be described as a solid, liquid or semi-solid substance used to grow various…

Q: Why is chlorination, although beneficial in terms of bacterial pathogen control, of environmental…

A: Chlorination is a disinfection procedure in which microorganisms are killed by the use of chlorine…

Q: You have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture.…

A: Answer : First step of the kirby-Bauer test :-

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.