Fig. 1 shows the possible effect of pH on recovery and impurity removal at the selection of a setpoint that allows for process control and enables a robust method. At what pH the recovery is at its highest and the impurities near their lowest. Also explains the intersection of two lines at pH 5.5. PROTEIN PURIFICATION METHODS 90 80 70 60 - Recovery (%) 50 -Impurity Concentration (ng/ml) 40 30 20 10 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 pH Recovery (%) or [Impurity] (ng/mL)

Fig. 1 shows the possible effect of pH on recovery and impurity removal at the selection of a setpoint that allows for process control and enables a robust method. At what pH the recovery is at its highest and the impurities near their lowest. Also explains the intersection of two lines at pH 5.5. PROTEIN PURIFICATION METHODS 90 80 70 60 - Recovery (%) 50 -Impurity Concentration (ng/ml) 40 30 20 10 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 pH Recovery (%) or [Impurity] (ng/mL)

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter28: Atomic Spectroscopy

Section: Chapter Questions

Problem 28.13QAP

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