Answered: Based on the given preparation…

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter25: Instruments For Optical Spectrometry

Section: Chapter Questions

Problem 25.20QAP

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Question

In order to improve the peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following:
“2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm3 in a graduated flask. To this solution, 50 cm3 of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm3 min-1.”
Based on the given preparation procedure, identify THREE mistakes that were made.

Wavenumber em
Spectrum (a)
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Spectrum (b)
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Spectrum (c)
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0-
Transmittance (%)
Transmitance (%)
Transmittance (%)

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