Figure la and 1b show two protein gels (SDS-PAGE) in which human blood serum has been electrophoresed. Albumin is the major serum protein and forms a heavy band. The al, a2 and B bands come from hemoglobin. The y band is IgG. Above each gel is a densitometry trace which quantitates the amount of protein in each band. The gel in Figure la is from a normal individual. One possible explanation is that the individual in Figure lb has a genetic disorder. What are two possible genetic changes that might lead to this result? Explain. Ibumin Albumin

Figure la and 1b show two protein gels (SDS-PAGE) in which human blood serum has been electrophoresed. Albumin is the major serum protein and forms a heavy band. The al, a2 and B bands come from hemoglobin. The y band is IgG. Above each gel is a densitometry trace which quantitates the amount of protein in each band. The gel in Figure la is from a normal individual. One possible explanation is that the individual in Figure lb has a genetic disorder. What are two possible genetic changes that might lead to this result? Explain. Ibumin Albumin

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter17: Genes And The Immune System

Section: Chapter Questions

Problem 17QP

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An MLS student on her clinical rotation is running an immunofixation electrophoresis procedure on a 49 yo. male. She notes something peculiar in the total serum protein lane or in some systems this is called the SPE lane. In this lane, there is no band where albumin should be. All of the other bands are present. She checks the patient’s chemistry profile to find that his serum albumin is in the normal range.1. Is albumin an anion or cation?2. Which direction should albumin move during electrophoresis, toward the anode or cathode?3. What possible reason or reasons would cause the albumin not to show up on the gel?4. Should the test be repeated and if so how should it be done differently?5. Is it possible that the patient’s Iiver does not produce albumin due to chronic liver disease and that is why it did not show up on the SPE?
An MLS student on her clinical rotation is running an immunofixation electrophoresis procedure on a 49 yo. male. She notes something peculiar in the total serum protein lane or in some systems this is called the SPE lane. In this lane, there is no band where albumin should be. All of the other bands are present. She checks the patient’s chemistry profile to find that his serum albumin is in the normal range.1. Is albumin an anion or cation?2. Which direction should albumin move during electrophoresis, toward the anode or cathode?3. What possible reason or reasons would cause the albumin not to show up on the gel?4. Should the test be repeated and if so how should it be done differently?5. Is it possible that the patient’s Iiver does not produce albumin due to chronic liver disease and that is why it did not show up on the SPE? P/S: part 1, 2, and 3 was solved
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