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- What is the significance that the combined absorption spectra of chlorophylls a and b roughly match the action spectrum of photosynthesis? Would photosynthesis be more efficient if their individual absorption spectra coincided exactly?compare and contrast the quantum yields of photochemistry and photosynthetic carbon fixation. Explain the differences between the respective quantum yield values.In cyclic photophosphorylation, it is estimated that two electrons must bepassed through the cycle to pump enough protons to generate one ATP.Assuming that the ∆G for hydrolysis of ATP under conditions existing inthe chloroplast is about -50 kJ/mol, what is the corresponding percent efficiency of cyclic photophosphorylation, using light of 700 nm?
- Why is a hypotonic buffer used for the resuspension? 1.DCPIP passes easily across the intact chloroplast membrane, but can't get into the thylakoid, so electron transport could not be reliably measured if using a hypotonic buffer. 2.To gently lyse the chloroplasts, leaving the electron transport membranes intact, whilst allowing DCPIP access 3.To gently separate the chloroplasts from each other, leaving the electron transport membranes intact, whilst allowing DCPIP access 4.DCPIP cannot easily across the intact chloroplast membrane, so electron transport could not be reliably measured if using a hypotonic buffer.In cyclic photophosphorylation, it is estimated that two electrons must be passed through the cycle to pump enough protons to generate one ATP. Assuming that the ΔG for hydrolysis of ATP under conditions existing in the chloroplast is about -50 kJ/mol, what is the corresponding percent efficiency of cyclic photophosphorylation, using light of 700 nm?What is the significance that the combined absorption spectra of chlorophylls a and b roughly match the action spectrum of photosynthesis? Wouldphotosynthesis be more efficient if their individual absorption spectra coincided exactly?
- Observing the over-all equation for photosynthesis, what are two variables other than light intensity, which would have an influence on how quickly the leaf disks float? Thanks in advanceIn addition, the proportion of each electron carrier (ferredoxin, NADP+,plastoquinone, cyt b559, cyt f, plastocyanin and cyt b6) that is oxidized duringphotosynthesis can be measured spectrophotometrically. From the data given below,identify each column with one of the electron carriers listed above, and give a shortreason to justify each choice. One of the electron carriers might not be represented by aletter. There may be ambiguity, where you can’t narrow down one letter between twosubstances – that’s fine, just explain why you can’t tell. The number shows the proportion of the lettered electron carrier that is oxidized; that is0.0 means none of that carrier is oxidized, and 1.0 means that it is fully oxidized. (attached table)A =B =C =D =E =F =Write the overall net reaction for photosyynthetic Co2 fixation. (Take into account both light and cark reaction of photosynthesis?)
- Is it reasonable to list standard reduction potentials for the reactions of photosynthesis? Why or why not?Calculate the energy cost of the Calvin cycle combined with glycolysis and oxidative phosphorylation, that is, the ratio of the energy spent synthesizing starch from CO2 and photosynthetically produced NADPH and ATP to the energy generated by the complete oxidation of starch. Assume that each NADPH is energetically equivalent to 2.5 ATP and that starch biosynthesis and breakdown are mechanistically identical to glycogen synthesis and break down.The net equation for oxidative phosphorylation can be written as 2NADH + 2 H+ + O2 →2 H2O + 2 NAD+. Write an analogous equation for the light reactions of photosynthesis.