F’ lac I- O+ Z+/ lac I+ Oc Z- the genotype indicated would lead to what type of expression of β‐galactosidase with respect to lactose inducibility of the lac operon. A. Constitutive B. Cannot be determined C. Inducible D. Uninducible

F’ lac I- O+ Z+/ lac I+ Oc Z- the genotype indicated would lead to what type of expression of β‐galactosidase with respect to lactose inducibility of the lac operon. A. Constitutive B. Cannot be determined C. Inducible D. Uninducible

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Description: F’ lac I- O+ Z+/ lac I+ Oc Z- the genotype indicated would lead to what type of expression of β‐galactosidase with respect to lactose inducibility of the lac operon. A.Constitutive B.Cannot be determined C.Inducible D.Uninducible

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...

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If β-galactosidase is expressed when lactose is absent, what does this suggest about the lac operon? a) There is a mutation in the repressor protein b) There is a mutation in the catabolite activator protein c) There is a mutation in the promoter region d) There is a mutation in the luxI protein
1. Assuming that the level of glucose is low, a mutation in the repressor associated with the lac operon of E. coli which prevents binding of the repressor to lactose should result in: a. constitutive expression of the lac operon genes b. lack of expression or refuced expression of the lac operon genes under all circumstances c. expression of the genes only when lactose is present d. expression of the genes only when lactose is absent e. lack of expression of the lac operon genes, resulting in an increase in lactose production
The lac operon controls lactose metabolism in bacteria. Predict the phenotype of a LacP mutation. • A. The lac genes would be expressed efficiently only in the absence of lactose. • B. The lac genes would be expressed efficiently only in the presence of lactose. • C. The lac genes would be expressed continuously. O D. The lac genes would never be expressed efficiently.
Gene expression in bacteria is controlled by the operon model. a) Why is the operon model important? b) Why is the trp operon known as a repressible operon?
Which of the following statements is false?a) A derivative of lactose serves as an inducer of the lac operon.b) Signal transduction provides a mechanism for a cell to sense the conditions of its external environment.c) Quorum sensing allows bacterial cells to sense the density of like cells.d) An example of a two-component regulatory system is the lactoseoperon, which is controlled by a repressor and an activator.e) An ORF is a stretch of DNA that may encode a protein.
There is Hyaluronic acid synthesis occuring in Group X Strep and it is controlled by an operon with 3 genes, called hasXYZ. Based on the 3-line diagram model, a. How many ribosome binding sites are there for the protein? b. How many promoters are there for the genes? c. How many start codons are there for the protein? d. How many RNA Polymerase binding locations are there for the genes? e. How many proteins will be fully functional? f. How many mRNA strands are made?
Operons A. Draw a schematic of the lac operon when glucose is low and lactose is high in the cell’s environment. B. Draw a schematic of the trp operon when tryptophan levels in the environment are low. C. What is the lux operon and how is affected by quorum sensing?
If tryptophan is absent from the environment of E.coli, the trp operon will be _______ A) mutated B) activated, but only partially C) activated D) repressed
The lac operon has 4 genes, I, Z, Y and A. For each scenario, tell me the result of the mutation, what would happen if this mutant was in the presence of lactose and why. A) Lac I is mutated/not functional - B) Lac Y is mutated/not functional -
Strain ROFL4 has a premature stop mutation in the lacZ gene, resulting in a nonfunctional b-galactosidase. Otherwise all other parts of the operon are functional. Circle the least number of components for an F' plasmid that will restore normal regulation and function of the lac operon in the resulting partial diploid. (may need more than one) (a) lacI+ (b) lacO+ (c) lacP+ (d) lacZ+ (e) None, cannot be restored.
The lactose operon in E. coli comprises two structural genes and three regulatory components.a.State the name of each structural gene and describe its function: b.) State the name of each regulatory component and describe its function
1) A. What is the difference between a repressible vs, an inducible operon? B. Using diagrams and words, describe how the Lac Operon is regulated by different levels of lactose and glucose. Be sure to show what’s happening at the molecular level, including the roles of lactose, glucose the repressor protein, cAMP and CAP.