5a. The table below summarizes expression of B-galactosidase (lacZ) and permease (lacY) from the lac operon, respectively. The strains are grown in the absence or presence of lactose. The “_“ indicates no/low expression and “+" indicates high expression. For each strain (A-G) write out one possible genotype (more than one might be possible) in the table for a lac operon that is consistent with the expression pattern. Do not give partial diploid genotypes. The first condition (upper right) is filled in for you as an example. Strain Lactose Absent Lactose Present B-galactosidase Permease B-galactosidase Permease Lac operon genotype(s) а. + lac+lacP+lacO+lacZ+lacY+ b. d. e. f. + + + g. +|+

Biology: The Dynamic Science (MindTap Course List)
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Chapter15: From Dna To Protein
Section: Chapter Questions
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5a. The table below summarizes expression of B-galactosidase (lacZ) and permease (lacY) from the lac
operon, respectively. The strains are grown in the absence or presence of lactose. The "_“ indicates no/low
expression and “+" indicates high expression. For each strain (A-G) write out one possible genotype
(more than one might be possible) in the table for a lac operon that is consistent with the expression
pattern. Do not give partial diploid genotypes. The first condition (upper right) is filled in for you as an
example.
Strain
Lactose Absent
Lactose Present
B-galactosidase Permease B-galactosidase
Permease
Lac operon genotype(s)
а.
+
+
lac+lacP+lacO+lacZ+lacY+
b.
d.
e.
f.
+
+
+
+
g.
+
4
5b. Assuming that the lac operon genotype in strain e contains a trans-acting mutation, would adding a
copy of lact on a plasmid be dominant or recessive to the existing mutation? Explain.
Transcribed Image Text:5a. The table below summarizes expression of B-galactosidase (lacZ) and permease (lacY) from the lac operon, respectively. The strains are grown in the absence or presence of lactose. The "_“ indicates no/low expression and “+" indicates high expression. For each strain (A-G) write out one possible genotype (more than one might be possible) in the table for a lac operon that is consistent with the expression pattern. Do not give partial diploid genotypes. The first condition (upper right) is filled in for you as an example. Strain Lactose Absent Lactose Present B-galactosidase Permease B-galactosidase Permease Lac operon genotype(s) а. + + lac+lacP+lacO+lacZ+lacY+ b. d. e. f. + + + + g. + 4 5b. Assuming that the lac operon genotype in strain e contains a trans-acting mutation, would adding a copy of lact on a plasmid be dominant or recessive to the existing mutation? Explain.
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