Fluorescence intensity was taken of 2.0 mL of the 10 μg/mL quinine standard solution into five 25 mL volumetric flasks. Into one, place 1.0 mL of 0.05 M sodium bromide solution and dilute to the mark with 0.05 M sulfuric acid solution. Similarly, add 2.0, 4.0, 8.0, and 16.0 mL of the sodium bromide solution to the remaining flasks before diluting to volume with the sulfuric acid solution. Why did the fluorescence intensity decrease as the concentration of sodium bromide increased?

Fluorescence intensity was taken of 2.0 mL of the 10 μg/mL quinine standard solution into five 25 mL volumetric flasks. Into one, place 1.0 mL of 0.05 M sodium bromide solution and dilute to the mark with 0.05 M sulfuric acid solution. Similarly, add 2.0, 4.0, 8.0, and 16.0 mL of the sodium bromide solution to the remaining flasks before diluting to volume with the sulfuric acid solution. Why did the fluorescence intensity decrease as the concentration of sodium bromide increased?

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter9: Atomic Absorption And Atomic Fluorescence Spectrometry

Section: Chapter Questions

Problem 9.21QAP

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