A spectrophotometric method for the quantitative determination of the concentration of Pb2+ in blood yields an Ssamp of 0.133 for a 1 mL sample of blood that has been diluted to 6 mL. A second sample is spiked with 1 µL of a 1467 ppb Pb2+ standard and diluted to 6 mL, yielding an Sspike of 0.491. Determine the concentration of Pb2+ in the original sample of blood.

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter34: Particle Size Determination
Section: Chapter Questions
Problem 34.13QAP
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A spectrophotometric method for the quantitative determination of the concentration of
Pb2+ in blood yields an Ssamp of 0.133 for a 1 mL sample of blood that has been diluted to 6
mL. A second sample is spiked with 1 µL of a 1467 ppb Pb2+ standard and diluted to 6 mL,
yielding an Sspike of 0.491. Determine the concentration of Pb2+ in the original sample of
blood.

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