Fluorescent FM dyes partition reversibly into biological membranes without penetrating through them. Suppose that you have neurons cultured in a dish. You incubate the neurons with an FM dye, and then you wash them with a medium to remove the dye. a) You find that if the neurons are stimulated to trigger action potentials during incubation with the dye, the synaptic regions of the neurons remain fluorescent after the wash. Propose an explanation for this effect. Due to moderate affinity of fluorescent dyes to lipid membrane and its lipid-sensitive emission, it can be readily loaded into recycled synaptic vesicles and released when those vesicles are exocytosed making the synaptic regions of the neurons fluorescent.
Fluorescent FM dyes partition reversibly into biological membranes without penetrating through them. Suppose that you have neurons cultured in a dish. You incubate the neurons with an FM dye, and then you wash them with a medium to remove the dye. a) You find that if the neurons are stimulated to trigger action potentials during incubation with the dye, the synaptic regions of the neurons remain fluorescent after the wash. Propose an explanation for this effect. Due to moderate affinity of fluorescent dyes to lipid membrane and its lipid-sensitive emission, it can be readily loaded into recycled synaptic vesicles and released when those vesicles are exocytosed making the synaptic regions of the neurons fluorescent.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Fluorescent FM dyes partition reversibly into biological membranes without penetrating through
them. Suppose that you have neurons cultured in a dish. You incubate the neurons with an FM dye,
and then you wash them with a medium to remove the dye.
G
a) You find that if the neurons are stimulated to trigger action potentials during
incubation with the dye, the synaptic regions of the neurons remain fluorescent after
the wash. Propose an explanation for this effect.
Due to moderate affinity of fluorescent dyes to lipid membrane and its lipid-sensitive emission, it can be
readily loaded into recycled synaptic vesicles and released when those vesicles are exocytosed making
the synaptic regions of the neurons fluorescent.
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Fluorescent FM dyes partition reversibly into biological membranes without penetrating through
them. Suppose that you have neurons cultured in a dish. You incubate the neurons with an FM dye,
and then you wash them with a medium to remove the dye.
G
a) You find that if the neurons are stimulated to trigger action potentials during
incubation with the dye, the synaptic regions of the neurons remain fluorescent after
the wash. Propose an explanation for this effect.
Due to moderate affinity of fluorescent dyes to lipid membrane and its lipid-sensitive emission, it can be
readily loaded into recycled synaptic vesicles and released when those vesicles are exocytosed making
the synaptic regions of the neurons fluorescent.
6
New Ta
F6
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3
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F7
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9
K
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