For the production of a secondary metabolic by Streptomyces coelicolor, a fed batch was performed. At the end of the single batch phase, the following conditions were reached in the reactor: V=10000L, cell concentration X=10g/L and product concentration P=0.1g/L. The feeding was then started with constant flow F= 200L/h, for 100h. Knowing that the substrate concentration in the feeding medium was SF= 80g/L and in the fermentation medium it was practically null, calculate: a) The final concentration of cells and product b) If the reactor were fed with a constant dilution rate (D), what should be the value of D used to reach the same cell concentration obtained in the situation with constant flow? μp= 0.01 g of product/ (g of cells.h) Y x/s = 0.15g cells/g of substrate
For the production of a secondary
a) The final concentration of cells and product
b) If the reactor were fed with a constant dilution rate (D), what should be the value of D used to reach the same cell concentration obtained in the situation with constant flow?
μp= 0.01 g of product/ (g of cells.h)
Y x/s = 0.15g cells/g of substrate
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