Forty grams of powdered banana pulp was mixed with 80.00 mL of cold Tris-HCl extracting buffer to isolate its protein content The protein content of the crude extracts was determined using a colorimetric Bradford assay with Bovine Serum Albumin (BSA) as standard. The standard BSA solutions were prepared by obtaining a certain volume of 2 mg/mL stock BSA solution an diluting it to 2 mL with deionized distilled water. The results of the experiment are as follows: Absorbance of 2 mL standard BSA solutions Prepared using 2 mg/mL stock BSA Absorbance of crude protein extract solution Volume of stock solution used, uL Absorbance at 595 nm Dilution Absorbance at 595 nm 0 0.000 Undiluted 4.286 200 0.170 1:2 2.022 400 0.314 1:5 0.785 600 0.500 800 0.684 1000 0.857 1. Determine the concentration of each standard solution (in mg/mL). 2. Determine the concentration of the sample (in mg/mL). 3. Calculate the total protein content (in grams) of the original sample.

Forty grams of powdered banana pulp was mixed with 80.00 mL of cold Tris-HCl extracting buffer to isolate its protein content The protein content of the crude extracts was determined using a colorimetric Bradford assay with Bovine Serum Albumin (BSA) as standard. The standard BSA solutions were prepared by obtaining a certain volume of 2 mg/mL stock BSA solution an diluting it to 2 mL with deionized distilled water. The results of the experiment are as follows: Absorbance of 2 mL standard BSA solutions Prepared using 2 mg/mL stock BSA Absorbance of crude protein extract solution Volume of stock solution used, uL Absorbance at 595 nm Dilution Absorbance at 595 nm 0 0.000 Undiluted 4.286 200 0.170 1:2 2.022 400 0.314 1:5 0.785 600 0.500 800 0.684 1000 0.857 1. Determine the concentration of each standard solution (in mg/mL). 2. Determine the concentration of the sample (in mg/mL). 3. Calculate the total protein content (in grams) of the original sample.

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter28: Atomic Spectroscopy

Section: Chapter Questions

Problem 28.13QAP

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