Four Hfr strains are derived from an F+ strain of E. Coli to serve as donors for an interrupted-mating experiment. Use the time-table and partial map of the F+ strain (shown below) to determine the genes’ respective positions. Keep in mind that the map distances are NOT proportional, only the FIRST 5 markers are indicated per strain, and the entry times, recorded in minutes, are in parentheses. Transferred genes represent wild-type alleles. Based on the data, which gene can be located at position 5 on the map?

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter3: Basic Hemostasis
Section3.6: D-dimers
Problem 8RQ
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Four Hfr strains are derived from an F+ strain of E. Coli to serve as donors for an interrupted-mating experiment. Use the time-table and partial map of the F+ strain (shown below) to determine the genes’ respective positions. Keep in mind that the map distances are NOT proportional, only the FIRST 5 markers are indicated per strain, and the entry times, recorded in minutes, are in parentheses. Transferred genes represent wild-type alleles. Based on the data, which gene can be located at position 5 on the map?

lac+
mal+
Strain Q
Mal (6')
Lac (20')
Cys (25')
Asn (37')
Glu (45')
1.
7.
Strain R
2.
Pro (11')
His (33')
Mal (43')
Lac (57')
Cys (62')
6.
3.
Strain S
Glu (4')
Asn (12')
Cys (24')
Lac (29')
Mal (43')
5.
4.
Strain T
Cys (2')
Asn (14¹)
Glu (22')
Val (32')
Ser (34')
Transcribed Image Text:lac+ mal+ Strain Q Mal (6') Lac (20') Cys (25') Asn (37') Glu (45') 1. 7. Strain R 2. Pro (11') His (33') Mal (43') Lac (57') Cys (62') 6. 3. Strain S Glu (4') Asn (12') Cys (24') Lac (29') Mal (43') 5. 4. Strain T Cys (2') Asn (14¹) Glu (22') Val (32') Ser (34')
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