Fraction no. Elution Vol. (mL) Abs (λ = 410) Fraction no. Elution Vol. (mL) Abs (λ = 410) 1 (PB pH 6.0) 1 0.0050 11 11 0.0041 2 2 0.0032 12 12 0.0060 3 3 0.0040 13 13 0.0064 4 4 0.0064 14 14 0.0063 5 5 0.0067 15 15 0.1109 6 6 0.0067 16 16 2.8902 7 7 0.0050 17 17 0.8500 8 (PB pH 8.0) 8 0.0049 18 18 0.2019 9 9 0.0052 19 19 0.0378 10 10 0.0050 20 20 0.0073 Use the data above to plot an elution profile, in which the absorbance is on Y-axis, and elution volume (volume = fraction number) is on X-axis. The elution profile must indicate at (1) which point the pH change occurred, (2) the fraction(s) which contain the highest myoglobin content, and (3) the maximum absorbance detected.
Fraction no. Elution Vol. (mL) Abs (λ = 410) Fraction no. Elution Vol. (mL) Abs (λ = 410) 1 (PB pH 6.0) 1 0.0050 11 11 0.0041 2 2 0.0032 12 12 0.0060 3 3 0.0040 13 13 0.0064 4 4 0.0064 14 14 0.0063 5 5 0.0067 15 15 0.1109 6 6 0.0067 16 16 2.8902 7 7 0.0050 17 17 0.8500 8 (PB pH 8.0) 8 0.0049 18 18 0.2019 9 9 0.0052 19 19 0.0378 10 10 0.0050 20 20 0.0073 Use the data above to plot an elution profile, in which the absorbance is on Y-axis, and elution volume (volume = fraction number) is on X-axis. The elution profile must indicate at (1) which point the pH change occurred, (2) the fraction(s) which contain the highest myoglobin content, and (3) the maximum absorbance detected.
Chapter7: Statistical Data Treatment And Evaluation
Section: Chapter Questions
Problem 7.13QAP
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Chemistry
|
Fraction no. |
Elution Vol. (mL) |
Abs (λ = 410) |
|
Fraction no. |
Elution Vol. (mL) |
Abs (λ = 410) |
|
1 |
(PB pH 6.0) 1 |
0.0050 |
|
11 |
11 |
0.0041 |
|
2 |
2 |
0.0032 |
|
12 |
12 |
0.0060 |
|
3 |
3 |
0.0040 |
|
13 |
13 |
0.0064 |
|
4 |
4 |
0.0064 |
|
14 |
14 |
0.0063 |
|
5 |
5 |
0.0067 |
|
15 |
15 |
0.1109 |
|
6 |
6 |
0.0067 |
|
16 |
16 |
2.8902 |
|
7 |
7 |
0.0050 |
|
17 |
17 |
0.8500 |
|
8 |
(PB pH 8.0) 8 |
0.0049 |
|
18 |
18 |
0.2019 |
|
9 |
9 |
0.0052 |
|
19 |
19 |
0.0378 |
|
10 |
10 |
0.0050 |
|
20 |
20 |
0.0073 |
Use the data above to plot an elution profile, in which the absorbance is on Y-axis, and elution volume (volume = fraction number) is on X-axis. The elution profile must indicate at (1) which point the pH change occurred, (2) the fraction(s) which contain the highest myoglobin content, and (3) the maximum absorbance detected.
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