Lisa is running an experiment to determine the kinetic parameters (Kmand Vmax) of the enzyme that she is studying. She has run a series of experiments where she changed the amount of substrate added and measured the accumulation of product over a 5 second time interval. She used this data to calculate the dependence of the rate of the reaction on substrate concentration. Her results listed below. Substrate Concentration (mM) Velocity (mM/sec) 1 2 5 2.5 4 6.3 10 7.6 20 1. Using this information construct a Michaelis-Menten curve. 2. Using this information construct a Lineweaver-Burk plot. 3. Using the Lineweaver-Burk plot determine the values of Km and Vmax.

Lisa is running an experiment to determine the kinetic parameters (Kmand Vmax) of the enzyme that she is studying. She has run a series of experiments where she changed the amount of substrate added and measured the accumulation of product over a 5 second time interval. She used this data to calculate the dependence of the rate of the reaction on substrate concentration. Her results listed below. Substrate Concentration (mM) Velocity (mM/sec) 1 2 5 2.5 4 6.3 10 7.6 20 1. Using this information construct a Michaelis-Menten curve. 2. Using this information construct a Lineweaver-Burk plot. 3. Using the Lineweaver-Burk plot determine the values of Km and Vmax.

Principles of Modern Chemistry

8th Edition

ISBN:9781305079113

Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Chapter18: Chemical Kinetics

Section: Chapter Questions

Problem 75AP

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