Genetically modified Atlantic salmon have recently been approved for human consumption. These genetically modified Atlantic salmon have a growth hormone gene from the Chinook salmon which allows them to grow to market size in double the time. This was possible because of CRISPR/Cas9 technology. From what you know about gene editing and CRISPR/Cas9, name 2 components of the technology that had to be introduced into the Atlantic salmon. Which method of DNA repair had to be used (non-homologous end joining or homology‐directed repair) to edit the Atlantic salmon genome?

Genetically modified Atlantic salmon have recently been approved for human consumption. These genetically modified Atlantic salmon have a growth hormone gene from the Chinook salmon which allows them to grow to market size in double the time. This was possible because of CRISPR/Cas9 technology. From what you know about gene editing and CRISPR/Cas9, name 2 components of the technology that had to be introduced into the Atlantic salmon. Which method of DNA repair had to be used (non-homologous end joining or homology‐directed repair) to edit the Atlantic salmon genome?

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 10SQ

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Now that you understand how the CRISPR-Cas9 system works, think back to the experiments discussed in the introduction to this chapter, in which researchers used CRISPR-Cas9 genome editing to treat mice with Duchenne muscular dystrophy. Why did the researchers choose to cut out the entire exon 23 in the mice with the disorder? Why not replace the specific mutation using a donor piece of DNA and homologous recombination? Propose some possible explanations.
Which of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer choices A single guide RNA (sgRNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence A single guide RNA (sgRNA) recognizes a genomic region followed by a long sequence palindrome repeat A single guide DNA (sgDNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence PAM sequences induce single stranded breaks that are then repaired by the CAS-9 enzyme
You are performing an experiment using CRISPR-cas9 to genetically modify the LacZ gene of a culture of E. coli. After you run the experiment, you decide to use gel electrophoresis to genotype the different bacterial cultures to determine if the gene editing was successful. How could your electrophoresis results confirm that the PCR was successful? And how could your electrophoresis results confirm that you successfully extracted genomic DNA from your bacterial samples?
Why does Northern blotting not require the use of restriction enzymes? a. Restriction enzymes cut at specific sites within DNA is this right?
A DNA library is a collection of clones, each containing a different fragment of DNA, inserted into a cloning vector. What is the difference between a cDNA library and a genomic DNA library?
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