GENETICS The modified "dye terminator method for DNA sequencing represented a major improvement over Sanger's original method because ( among other things) it does NOT require a) a DNA primer b) DNA polymerase c) the use of four separate sequencing reactions for each template d) the use of electrophoresis to separate DNA chains based on size e) the used chemically modified dNTPs f) all of the above

GENETICS The modified "dye terminator method for DNA sequencing represented a major improvement over Sanger's original method because ( among other things) it does NOT require a) a DNA primer b) DNA polymerase c) the use of four separate sequencing reactions for each template d) the use of electrophoresis to separate DNA chains based on size e) the used chemically modified dNTPs f) all of the above

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1TYK

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Similar questions

During PCR amplification in preparation for DNA sequencing, why were there different colors at the 3’ ends of the fragments produced? (What did these four colors represent?)
Assuming that you start with one genome copy and are trying to amplify a 2000 bp DNA fragment, how many rounds of PCR do you need to do before you have products of the correct length?
In next-generation sequencing, which of these advances allows for massively parallel sequencing? a. Pieces of DNA are fixed to a surface, so we can tell which new nucleotides were added to each piece. b. DNA sequences are read in real-time as nucleotides are added to each piece. c. Each segment of the genome can be pieced back together through shotgun alignment d. Single molecules of DNA can be read without the need for amplification.
Which of the DNA polymerases shown in Table have the ability to proofread?
What are the disadvantages of doing DNA sequencing?
Which of the following is true regarding next generation sequencing? 1. Next generation sequencing is also known as sequencing by synthesis 2. Next generation sequences uses DDNTPS to identify the base at the end of the nucleotide chain 3. Next generation sequencing will not work if chain terminating nuclear tides are included in the reaction 4. Next generation sequences uses fluorescently labeled ddNTPs instead of radioactivity labeled ddNTPs 5. Next generation sequencing allows hundreds of millions of DNA fragments to be sequenced at the same time
Suppose you have been directed to find new enzymes to use in the breakdown of wood in order to process biofuel (switchgrass, for example). Suppose you wanted to use fungal or bacterial DNA from the environment in order to do so. DNA can be unwound from the double stranded double helix into single strands, amplified, separated on gels by size, stained with dyes. It can be mutated by a variety of means. It can be sequenced. Describe one or more of the ways that you might manipulate DNA towards the stated goal. Relate the technology you plan to utilize to the structure of DNA. (You can break this into multiple posts, as multiple procedures might be used).
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