How did Kornberg assess the fidelity of DNA polymerase I in copying a DNA template?

Q: What is transformation? Describe Grifith’s experiment to show transformation? What did he prove from…

A: Bacteria contain a single circular chromosome which is their genetic material. It lies within the…

Q: What are DNA excision-repair systems?

A: Molecular biology is the field of biology that studies the composition, structure, and interactions…

Q: How does size of DNA affect size traveled during electrophoresis?

A: Electrophoresis is a technique for the separation followed by the analysis of the macromolecules…

Q: What are STRs, and why are they useful for DNA profiling?

A: Introduction  In the genome there are usually two type of nucleic acid sequence present. One is Non…

Q: What are the five main steps in DNA cut-and-paste transposition?

A: The "cut and paste" method is used for saving conversion. The enzyme transposase acts as a pair of…

Q: What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that…

A: Deoxyribonucleic acid or DNA is a nucleic acid that composed of two polynucleotide chain that is…

Q: Can you explain what semiconservative means in relationship to the way DNA is copied?

A: In the process of DNA replication, two daughter DNAs are formed with one old and one new strand…

Q: Outline the DNA extraction steps?

A: The extraction of DNA is an important initial step in the experiments of recombinant DNA technology.…

Q: Why Sanger Sequencing Dependson DNA Polymerase?

A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…

Q: What information do learn from the results of the DNA extraction?

A: 1.Breaking cells open to release DNA. 2. Separating DNA from proteins and other cellular debris.…

Q: What happens if DNA polymerase fails to correct an error?

A: DNA is a nucleic acid that serves to store genetic information.

Q: What is the mom method of pacaking DNA?

A: Answer: DNA : Deoxyribonuleic Acid acts as the genetic material which transfers the genetic…

Q: What is meant by “proofreading” with respect to DNA polymerase?

A: DNA polymerase belongs to the member of the family of enzymes that play the role in catalyzing the…

Q: How does gel electrophoresis separate different-sized DNA fragments?

A: Gel electrophoresis is a molecular biology technique used to separate macromolecules like DNA, RNA…

Q: The purpose of using a washing soap in DNA extraction experiment is to

A: Washing soap is made of salts of fatty acids which dissolves the plasma membrane by solubilization…

Q: How was the first Copy Cat was cloned from a kitten?

A: Cloning is the practice of creating organisms, either spontaneously or intentionally, with similar…

Q: How does proofreading take place in the process of DNA replication?

A: DNA replication is the process of producing two identical replices of DNA from one original DNA…

Q: How New Techniques Sequence Millions ofIndividual DNA Molecules in Parallel?

A: Massive parallel sequencing is a high throughput approach to DNA sequencing by using the concept of…

Q: Describe the Meselon -Stahl experiment  Include how they distinguished new from old DNA strands,…

A: Meselson and Stahl Experiment In 1958, Matthew Meselson and Franklin Stahl performed an experiment…

Q: does Sanger sequencing still used?

A: Sanger sequencing is a first generation DNA sequencing method. Despite the advantage of next…

Q: What special kind of nucleotide is used in the Sanger sequencing procedure?

A: A British biochemist named Fred Sanger and few of his research colleagues developed the process of…

Q: For PCR, what property of DNA does temperature influence? Why do the “denaturation” and “annealing”…

A: PCR stands for polymerase chain reaction. It is an in-vitro laboratory technique used for generating…

Q: Explain the Powerful Technique for Copying DNA ?

A: Introduction: Multiple copies of DNA can be synthesized in the in-vitro medium with the help of the…

Q: What is the purpose of adding the dishwashing liquid to the DNA extraction?

A: DNA extraction is a method of separating DNA from cell membranes, proteins, and other cellular…

Q: What is a read (Sanger sequencing)?

A: DNA sequencing is the process of determining the sequence of nucleotides (adenine, guanine,…

Q: how are okazaki fragments created?

A: An Okazaki fragment refers to DNA fragments that are formed during the DNA replication process.

Q: What key structural features of the DNA molecule underlie its ability to be faithfully replicated?

A: DNA is known as deoxyribonucleic acid. DNA is the genetic material in humans and other organisms.…

Q: How can specific genes be retrieved from a DNA library?

A: Deoxyribonucleic acid or DNA is a molecule that contains the genetic code of the organisms. DNA…

Q: Why is the use of temperaturestable DNA polymerase an important factor in the polymerase chain…

A: The polymerase chain reaction (PCR) is the process of artificially synthesizing multiple copies of a…

Q: Gel Electrophoresis Separates DNAFragments According to which thing?

A: Electrophoresis is a technique used for separating the components of a mixture of charged molecules…

Q: What did the Watson–Crick model suggest about the replication of DNA?

A: The most exciting aspect of the structure of DNA deduced by Watson and Crick was, as expressed in…

Q: What is the enzyme responsible for methylating DNA? How does a drug like doxorubicin affect DNA…

A: DNA is the nucleic acids present in the organisms. DNA is the deoxy ribose nucleic acid in which…

Q: . Explain how gel electrophoresis is used to separate DNA fragments of different lengths.

A: Gel electrophoresis is used for the separation and analysis of the micromolecules and their…

Q: Describe how certain restriction enzymes generate DNA fragments with sticky ends, and others…

A: Restriction enzymes also called molecular scissors are the type of nuclease enzymes that cleaves the…

Q: What is a direct way of repairing double-stranded DNA breaks?

A: DNA is the genetic material of all living cells. DNA forms a double-stranded double helix. The two…

Q: What is RNA primer?

A: The nucleic acid is a nucleotides chain that stores genetic information. It forms RNA and DNA that…

Q: What is a processive enzyme? Explain why processivity is an important feature of DNA polymerase.

A: Enzymes are substances produced by living organisms to carry out reactions at a high rate without…

Q: What is the results of extracting the DNA of a strawberry?

A: Ripe strawberries are an excellent source for extracting DNA because they are easy to pulverize and…

Q: What information do learn from the results of the DNA extraction?

A: Hi! Since you posted more than one question, we will be answering one for you. If you want the other…

Q: What are the three types of DNA ends that can be generated after cutting DNA with restriction…

A: A restriction enzyme, also known as a restriction endonuclease, is a bacterial protein that cleaves…

Q: How does proofreading differ from later DNA repair mechanisms in terms of timing and the proteins…

A: Proofreading done by DNA polymerase.DNA polymerase detect error at the time of replication and also…

Q: If insert DNA was obtained by PCR using Taq DNA polymerase, what shape does the end of this DNA…

A: Taq polymerase is basically a thermostable polymerase that is obtained from the bacteria called as…