Which of the following most accurately describes the process of DNA cloning? set of laboratory procedures that consist of cutting a segment of DNA with restriction enzymes set of laboratory procedures that uses living cells to mass-produce specific DNA fragments set of laboratory procedures by which a DNA fragment is transferred from a living organism to a SNP chip the manipulation of DNA fragments in a laboratory using modern techniques of molecular biology set of laboratory procedures that consist of isolating of a DNA fragment from a living organism and inserting it into a plasmid

Which of the following most accurately describes the process of DNA cloning? set of laboratory procedures that consist of cutting a segment of DNA with restriction enzymes set of laboratory procedures that uses living cells to mass-produce specific DNA fragments set of laboratory procedures by which a DNA fragment is transferred from a living organism to a SNP chip the manipulation of DNA fragments in a laboratory using modern techniques of molecular biology set of laboratory procedures that consist of isolating of a DNA fragment from a living organism and inserting it into a plasmid

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 7QP: Which of the following is NOT an activity carried out in the field of bioinformatics? a. collecting...

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The following statements are true about common gene cloning procedures except: -DNA plasmids can help move around genes into other cells - Restriction enzymes are very important for cutting up and linking chunks of DNA -We make it so that genes from plants or animals are expressed in bacteria so the products can be harvested - DNA plasmids are chunks of chromosomal DNA used for cloning
You want to identify and compare the gene expression profile of several genes in a disease. Will you be able achieve this using Q-PCR technique? Can you identify a potential mutation (the nucleotide change) within a gene sequence using conventional PCR? If not, then what approach you should take?
A student carries out PCR using the following steps: step 1: 94C for 1 minuteStep 2: 60C for 30seconds Step 3: 72C for 30 seconds The student is examining a 1000-bp DNA fragment as part of their research project. If the limit of detection of this molecule 9 x 108 molecules, what is the minimum number of PCR cycles you would have to run to detect PCR product generated from a single molecule of the template? 2. Two double-stranded fragments of DNA are exactly the same length. At 89 C, fragment A has completely denatured which means the two strands have separated. At that temperature, fragment B is still double-stranded. How might these fragments differ to result in different denaturation temperatures?
Why is it important to sequence positive clones derived from PCR cloning? Group of answer choices Errors could have been incorporated during restriction enzyme digestion and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during plasmid DNA extraction and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during PCR and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during cloning and so it is important to verify that only the expected protein is produced.
Herbert Boyer and Stanley Cohen pioneered the technique of DNA cloning allowing genes to be transferred from another biological species easily. Their work also gave rise to the development of different recombinant proteins with therapeutic applications like insulin and growth hormone. The former was cloned using Escherichia coli. coli in 1978. With this breakthrough, the first licensed drug produced using recombinant DNAtechnology was human insulin, developed by Genentech, licensed and marketed by Eli Lilly in 1982. Scientists were able to identify and isolate the gene fragment or the gene of interest, in this case, the gene that is responsible for producing insulin. Moreover, they were able to isolate the bacterial DNA of E. coli. The plasmid and DNA fragment were cut using a restriction enzyme. This DNA fragment was inserted into the plasmid using a DNA ligase. When the DNA fragment was then placed into the bacterial DNA, it was then introduced to the host cell (E. coli) and was then…
18) Ramachandran plot (how to read it) 19 )Rotations around bonds in a peptide sequence 20) DNA and RNA based techniques 21) Difference between DNA and RNA 22) PCR 23) RT-PCR 24) DNA synthesis and sequencing 25) Restriction enzymes 26) Techniques to determine gene function 27) SDS-PAGE 28) Reading DNA, RNA and protein sequences 29) Cloning vectors 30) Stereoisomerism and amino acids 31) Protein and nucleotide based electrophoresis and blotting 32) Centrifugation
Which of the following is true regarding next generation sequencing? 1. Next generation sequencing is also known as sequencing by synthesis 2. Next generation sequences uses DDNTPS to identify the base at the end of the nucleotide chain 3. Next generation sequencing will not work if chain terminating nuclear tides are included in the reaction 4. Next generation sequences uses fluorescently labeled ddNTPs instead of radioactivity labeled ddNTPs 5. Next generation sequencing allows hundreds of millions of DNA fragments to be sequenced at the same time
You are performing an experiment using CRISPR-cas9 to genetically modify the LacZ gene of a culture of E. coli. After you run the experiment, you decide to use gel electrophoresis to genotype the different bacterial cultures to determine if the gene editing was successful. How could your electrophoresis results confirm that the PCR was successful? And how could your electrophoresis results confirm that you successfully extracted genomic DNA from your bacterial samples?
In conventional PCR applications, a heat-resistant polymerase is used. Why? You want to identify and compare the gene expression profile of several genes in a disease. Will you be able achieve this using Q-PCR technique? Can you identify a potential mutation (the nucleotide change) within a gene sequence using conventional PCR? If not, then what approach you should take?
t is possible to buy a CRISPR kit that you can do in your own home. Is this a good idea? After watching the two CRISPR videos answer the following questions in your post. When is a gene editing technology safe and effective enough to be used outside of the laboratory? How do we balance risks and benefits appropriately? How far should we go in altering the future genetic landscape of free-living species, including humans? And importantly, who should decide such issues?
Practical applications of recombinant DNA technology include the: 1. Efficient production of useful proteins and development of a new type of vaccines 2. Creation of novel genotypes for the synthesis of economically important molecules. 3. Generation of DNA and RNA sequences for use in medical diagnosis. 4. all of the above WHICH OF THE FOLLOWING TECHNIQUE IS NOT USED TO MANIPULATE THE GENOME 1. siRNA 2. ZF (zinc finger) 3. TALE (transcription activator light effector) 4. CRISPR COMMON DISEASE- COMMON VARIANT HYPOTHESIS STATES THAT 1. common disorders are likely influences by genetic variation that is also common in the population 2. if an SNP produced a deleterious mutation and changes amino acid sequence in 40% of individuals- it will produce disease phenotype in 40% individual 3. A SNP produce little change in gene expression- it will produce slight increase in risk/ manifestation of disease phenotype 4. all of the above WHAT IS TRUE REGARDING GENE ORGANIZATION IN THE NUCLEUS…
Which of the following best describes the complete sequence of steps occurring during every cycle of PCR? I-The primers hybridize to the target DNA. II-The mixture is heated to a high temperature to denature the double stranded target DNA. III-Fresh DNA polymerase is added. IV-DNA polymerase extends the primers to make a copy of the target DNA. A) II, I, IV. B) I, III, II, IV. C) III, IV, I, II. D) III, IV, II. E) II, III, IV.