Give an example of an industrial/clinical setting where quantifying viable bacteria would be useful. How would you prepare a series of dilutions to get a final dilution of 10–10? Outline each step.
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- Give an example of an industrial/clinical setting where quantifying viable bacteria would be useful.
How would you prepare a series of dilutions to get a final dilution of 10–10? Outline each step.
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- Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE DILUTIONS in series? Consider the accuracy of the pipetting so that you don’t make a single dilution that is too large.A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 223 colonies of bacteria grow on the petri dish. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.Describe three problems associated with using the standard plate count method for determining the number of bacteria in a sample.
- What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?If you obtain 35 colonies after spread plating 1 ml of a 1:500 dilution of lake water, what is the concentration of bacteria in the lake water?Compare and contrast bacteriocidal, bacteriostatic and bacteriolytic agents. What are their effects on the optical density (OD) and viable count of a bacterial culture, respectively?
- What is the importance of describing the morphological characteristics of bacterial colonies when cultures were sampled from an environmental source? Explain and justify your points.1 mL of a juice sample is taken and diluted in 9 mL culture medium, subsequently, this suspension is analyzed in the Petroff-Hausser chamber, determines that the total number of bacteria found is 44 bacteria per square, if the chamber has 25 squares, how many bacteria are in the chamber? initial juice sample?Make an outline about culture-dependent and culture-independent methods in microbiology and explain it in detail. Include all the topics, subtopics, and examples involved in the two methods, respectively.
- If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a suspension with 5 x 102 cells per ml? Show your answer using a serial dilution scheme or diagramYou perform a quadrant streak and isolate a single colony which contains two distinct species of bacteria. You repeat this with the same results. Provide a possible explanation for this result that does not involve contamination of the sample.can you Describe in 1500 words, the process of segregation of Bio-Medical wastes in a hospital?