Given the active site diagram below, which circled component coordinates the cofactor? 1 05 02 5 NH 2 *H₂N. Mn²+ HN H₂ Mn²+ -3
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- In the table below, name each functional group in anserine that is ionizable and record an estimated pKaA simple enzymatic reaction proceeds as follows. E+S↔E.S→ E+P Derive the Michaelis–Menten equation for this reaction.H159 functions as general acid. What are the catalytic function of C25? (shown in figure)
- Given the following information about an enzymatic reaction, calculate the initial velocity. Your numerical answer is assumed to have the units of nM sec-1. Vmax = 5 nM sec-1 KM = 1 mM [S] = 0.25 mMAssume you have an enzyme that catalyzes a reaction that breaks down dopachrome. At t = 0 s, the absorbance at 475 nm is 0.2 when you add the enzyme. At t = 30 s, would you expect the absorbance to be less than or greater than 0.2?Why is the overall coupled reaction exergonic?
- Give a complete and well descriptive definition of the following:1.1 Enzyme catalysis1.2 Co-enzyme1.3 Negative heterotropic co-cooperativitConsider the following chemical equation whose delta(G) = 9kcal/mol: AC + BD ---> AB + CD what are the reactants and what are the products is this reaction spontaneous? How do you know? Is energy released or consumed by this reaction? If an enzyme, which catalyzes this reaction is added, what will happen to delta (G) If this reaction is coupled to another reaction, whose delta(G) is -12 kcal/mol, what will be the net delta(G) value? will the overall reaction be spontaneousHow many moles of active sites are there in 1 mg of enzyme? Assume that each subunit has one active site.
- Given the following choices,a. what is the structure of the product of the reaction catalysed by ACAT?b. structure of the product produced from the reaction catalysed by phospolipase A2?c. general structure of TAGd. structure of glycerolUnder standard conditions, will the following reaction proceed spontaneously as written? Fumarate + NADH + H+⇌ succinate + NAD+Name four differential reactions that TSIA shows.