Tyrosinase enzyme activity is assayed by monitoring the oxidation of 3, 4-dihydroxyphenylalanine (dopa) to the red-colored dopachrome. a) Calculate the tyrosinase activity (U/mL) by using the experimental data given below: 1 ε = 3600 M¹ cm ¹ (molar absorption coefficient for the product dopachrome) v = 0.004 L (final volume in the cuvette)
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- Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Using the data in the first and third columns of the table below, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase and explain why.True or False. Do all reducing sugars produce a positive result in an Osazone test?Serum blood of a patient with dislipoproteinemia type 1 has milky appearance even in fasting. If serum stays at low temperature (40) for several hours fatty layer appears on its surface. What are the possible causes of these symptoms? To explain this, answer the questions and do the following tasks: a) what compounds of serum must be tested for that patient in biochemical lab? b) write the reaction which does not occur properly in patient’s blood; c) write down the schemes, explaining how the products of the previous reaction are used in adipose tissue and heart in healthy person 2 hours after a meal.
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