10. Escherichia coli is a bacteria that can cause food poisoning if people eat contaminated food or drink. In the laboratory, Escherichia coli can triple every hour. If the bacteria count in a sample contaminated water is found to be 50 after the first hour, how long will it be until the count exceeds 100 000? Express your answer in hours. Use the explicit formula for your solution. Express your answer to two decimal places. Use methods learnt in class.
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- 8. All the followings can be produced through Industrial Microbiology techniques except: a) antibiotics b) Amino Acids c) steroids d) polysaccharides19. The new process for cleaning the environment through bioorganic method using bacteria which genome has been modified is called: a) Bioconversion b) Biotechnology c) Bioinformatics d) BioremediationA sample of raw ground beef initially contained 25 E. Coli bacteria. After six hours, there are 102 400 cells in the sample. What is the growth rate of the E. coli population? Show all work. Express your answer using scientific notation in the form of xyz 10 d #bacteria/hr.1. The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization? 3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distilled
- 2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.)1. What is the importance of using a test/control organism in describing the cultural characteristics of an unknown bacterium? Explain your answer comprehensively and please, do not just copy from somewhere,6a. A sample is given to you that contains 6 million cells per ml. You must make a series of serial dilutions in order to get a countable number on a plate to prove that there are indeed 6 million cells per ml. The plate that you eventually put a sample of bacteria on must have between 10 and 100 colonies for ease of counting. What dilutions do you make to get this many colonies on a plate? How many colonies do you have on that plate? Show what dilutions you make (Write exactly how much liquid you move into each test tube and how much liquid was in the test tube, ex. Move 1 ml of culture into 9 ml of water – 10x dilution). Don’t forget to tell me how much liquid you eventually put on your plate. (If you want to draw something out, you could draw then take a picture of it with your phone and submit the image along with the worksheet). 6b. Do the same problem as in 6a, but now you have to do it in 3 test tubes or less. What dilutions do you do now? please use drawings to help…
- 1. Why do you think the 5% sheep blood agar was selected for the culture of mouth bacteria?the scientist is studying the effect of water purification tablets on the creek water in her local area. She collected several different 2 L samples from a creek. For each sample, she measured the number of coliform group bacteria colonies in the water initially, and then added a water purification tablet and measured the number of coliform group bacteria colonies over time. She found that the following equation was a good fit to the data: C(t) = 80e−0.05t + 8(t + 1)−0.5.Her model says that C(t) is the number of coliform group bacteria colonies at time t, where t is measured in minutes since the tablet was added to the water. (a) According to this model, how many coliform group bacteria colonies would initially be in a 2 L sample of the water? background information: The quality of drinking water is determined by tests for coliform group bacteria, which are organisms found in soil and in the intestinal tract of warm-blooded animals. Coliform group bacteria are not considered to…9. The process in which all organisms and pathogens are destroyed is called ------. a) Disinfection b) Sterilization c) Antisepsis d) Pasteurization
- The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization?3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distille1 The following bacteria are gram positive bacilli EXCEPT : A. Corynebacterium sp. B. Citrobacter C. Listeria sp. D. Lactobacillus sp. E. Clostridium sp.1) would you describe the contents of the soil-inoculated broth as being a “pure culture”? Why or why not? 2) How did the uninoculated broth differ in appearance from the broths inoculated with E. Coli and M. Luteus? And then how could you tell if a supposedly sterile, uninoculated broth was contaminated? Please explain in detail and highlight the important parts cuz I am confused and need help! Thanks