Growth of an E. coli bacteria in batch culture is observed. The bacteria are fed with 0.5 g / L glucose. Bacteria's abs value is measured as 0.133 ABS at the 0th minute, 0.152 ABS at the 30th minute, 0.215 ABS at the 60th minute, 0.23 ABS at the 90th minute, 0.256 ABS at the 120th minute, 0.286 ABS at the 150th minute and 0.305 ABS at the 180th minute. Calculate the specific growth rate of the bacteria accordingly.

Growth of an E. coli bacteria in batch culture is observed. The bacteria are fed with 0.5 g / L glucose. Bacteria's abs value is measured as 0.133 ABS at the 0th minute, 0.152 ABS at the 30th minute, 0.215 ABS at the 60th minute, 0.23 ABS at the 90th minute, 0.256 ABS at the 120th minute, 0.286 ABS at the 150th minute and 0.305 ABS at the 180th minute. Calculate the specific growth rate of the bacteria accordingly.

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 45SST

See similar textbooks

Similar questions

If the temperature of the incubator were to be increased from 35 to 45°C, how would this affect the bacterial growth curve of E. coli?
If the temperature of the incubator were to be increased from 35 to 45°C, how would this affect the bacterial growth curve of E. coli? (optimum temperature 20o-40oC; most human pathogens)
A slant is inoculated with 100 E.coli cells. The slant is left at room temperature where the generation time is 1 hour. How many E. coli are on the slant in 6 hours? If the slant was initially put in the 37C incubator, with a 20 minute generation time, how many more cells would have grown in the same time period?
Consider the table below. What is the TDT for E. coli at 70 C? Please include the unit.
The Kirby-Bauer test examines the effectiveness of antibiotics and disinfectants or antiseptics on separately plated bacteria. Which of the following Zones of Inhibition (ZOI) measurements on a plate of Klebsiella pneumoniae shows the strongest inhibition of the bacterium's growth? a) Penicillin, 7 mm ZOI b) Actinomycin, 9 mm ZOI c) Tetracycline, 26 mm ZOI d) Chloramphenicol 2 mm ZOI e) Streptomycin, 23 mm ZOI
Lysine decarboxylase media is yellow after incubating an organism in it at 37 degrees for 24 hours. Which of the following can be interpreted from this result? A. The organism did not grow so lysine decarboxylase activity could not be determined. B. The organism is negative for lysine decarboxylase. C. The media is too acidic to determine lysine decarboxylase activity. D. The organism is positive for lysine decarboxylase
One ml of a sample was added to 99 ml of buffer. 100 µ of this was plated in nutrient agar. After incubation, 247 colonies were present on the plate. How many colony-forming units were present per ml of the original sample? Show your working out. What would you record in your workbook if there were 378 colonies present? Why? B. Give definitions of these bacterial growth in relation to O2 availability. 1. E. coli , micrococcus Letues, clostridium sprogenes
If a bacteria is inoculated onto milk agar and an area of clearing is observed around the bacterial growth, the bacteria must be positive for the enzyme__________
A. Antibiotics, such as gentamicin, are used in media to prevent growth of susceptible microbes. Is gentamicin media a selective, differential or enrichment media? B. Sorbitol is a sugar alcohol that all Escherichia bacteria can normally ferment into an acidic waste compound. This acidic end product is detected by including a pH indicator in the agar and noticing a color change. The strain of Escherichia that is a common food pathogen, E. coli O157:H7, however can not ferment sorbitol. It will grow similar to other E. coli, but not change the agar color. Is sorbitol in nutrient agar a selective, differential or enrichment media?
Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________
Compute the cfu/g (lactic acid bacteria and coliforms) of these samples given the following values below. Show your solution. Media First Sampling First Sampling First Sampling Second Sampling Second Sampling Second Sampling 10-2 10-3 10-4 10-2 10-3 10-4 VRBA TNTC, TNTC 243, 232 24,22 23, 25 0, 0 0, 0 MRS 32, 40 0, 0 0, 0 TNTC, TNTC TNTC, TNTC 102, 120
A new intestinal isolate of E. coli is known to have a generation time of 12 minutes. If a culture contained 3, 221,225,472 bacterial cells after 6 hrs how many bacteria must have been inoculated into the culture at time 0.