Which of the following is true regarding microbial tests that lead to color change in tubes used to characterize bacteria? (more than one answer may be correct) OUrea is degraded by urease, causing a rise in pH O Urea is degraded by urease, causing a rise in acid level O Production of carboxylic acids causes a rise in pH O Production of carboxylic acids causes a drop in pH
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- A microbiologist is working with a pure strain of bacteria isolated from a mixed sample. Which of the following would indicate that the bacterium is NOT Helicobacter pylori? Cells have flagella when viewed by flagellar staining. Secretes acids produced from metabolism of sucrose. Cells are curved rods. Produces and secretes urease enzyme. Cells stain Gram negative.From a soup sample, 1 mL of sample is taken and diluted in 99 mL of soup medium. culture, later, this suspension is analyzed in the Petroff-Hausser chamber, It is determined that the total number of bacteria found is 53 bacteria per square, if the chamber has 25 squares, how many bacteria are found in the chamber? initial juice sample?Hello, I want you pleaseeee to make a dichotomous key based on provided experiments to identify a an unknown bacterium mixture. It can be E. coli, B. Bulgaris, B subtilis, b aerogenes, m leuteus, s. Aureus, and s epidermisis. Make it based on these possible experiments: phenol red broth, mr/vp test, catalase test, oxidase test, nitrate reduction test, citrate utilization test, Malonate utilization test, decarboxylation test, starch hydrolysis test, dna hydrolysis test, lipid hydrolysis test, gelatin hydrolysis test, urea hydrolysis, SIM medium, triple sugar iron agar test, blood agar test. So far we have run a MAC agar and PEAA plate of the bacterium
- Besides being able to tolerate a range of pH some bacteria change their environment by producing acid or alkali substances. How would you use microbiological media to determine a change in the pH of culture media due to metabolic activities of bacteria? GIVE DETAILED ANSWER PLEASEA microbiologist is working with a pure strain of bacteria isolated from a mixed sample. Which of the following would indicate that the bacterium is NOT Streptococcus mutans? Cells are arranged in chains. Secretes acids produced from metabolism of sucrose. Cells are cocci. Can grow in biofilms. Cells stain Gram negative.When you interpret a Gram-stained smear, you should also describe the morphology (shape) of the cells, and their arrangement. In the figure below, there are two distinct types of bacteria, distinguishable by Gram stain reaction, and also by their shape and arrangement. Below, describe these characteristics for both bacteria: Gram positive bacterium Gram negative bacterium Morphology cocci bacillus Arrangement
- The Kirby-Bauer test examines the effectiveness of antibiotics and disinfectants or antiseptics on separately plated bacteria. Which of the following Zones of Inhibition (ZOI) measurements on a plate of Klebsiella pneumoniae shows the strongest inhibition of the bacterium's growth? a) Penicillin, 7 mm ZOI b) Actinomycin, 9 mm ZOI c) Tetracycline, 26 mm ZOI d) Chloramphenicol 2 mm ZOI e) Streptomycin, 23 mm ZOIThe rapid identification methods used in this activity are designed to identify __________. Gram-negative, oxidase-negative bacteria Gram-negative bacteria aerobic bacteria Gram-positive bacteria all bacteriaWhich of the following statements regarding microbial control agents is FALSE? Question options: strongly acidic or alkaline disinfectants are typically more effective than neutral pH agents the presence of extracellular fats typically decreases the effectiveness of control agents a warmed disinfectant is typically more effective than the same disinfectant at room temperature substances secreted by members of biofilms increase protection against microbial control agents all of these statements are TRUE bacterial endospores are more resistant to antimicrobial treatment than vegetative bacterial cells
- In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: The primary stain, crystal violet, is dark purple The counterstain (safranin) causes the purple color to develop Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol Crystal violet is fixed inside the cell as a CV-Iodine complex once iodine is added None of the other four answers (All are reasons that gram-positive bacteria are purpleIn this activity, you will receive an “unknown” containing two species. The first step in identifying these bacteria is to __________. In this activity, you will receive an “unknown” containing two species. The first step in identifying these bacteria is to __________. inoculate a glucose fermentation tube perform an acid-fast stain streak a TSA plate for isolation inoculate two TSA slants inoculate two OF-glucose tubesWhich of the following media selects for gram negative bacteria by inhibiting the growth of gram positives that typically incorporate its dye component into their relatively bigger cell walls? blood agar EMB Mannitol salt agar Simmon citrate agar urease broth