the reagents were added and the results were recorded early, at 24 hours rather than at the full incubation time at 48 hours, would the color result for the VP (+) bacteria be the same at 24 hours than at 48 hours? If different, explain how the color result is different. What chemical would be missing at 24 hours that is needed for a positive result?

the reagents were added and the results were recorded early, at 24 hours rather than at the full incubation time at 48 hours, would the color result for the VP (+) bacteria be the same at 24 hours than at 48 hours? If different, explain how the color result is different. What chemical would be missing at 24 hours that is needed for a positive result?

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 10SST

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The ingredients in the MR/VP Broth are: Peptone (protein) 7g Glucose 5g Phosphate buffer 5g Distilled water 1L Examine the above figure. This figure shows how the pH in the Methyl Red and Voges-Proskauer (MR-VP) broth changes during incubation from time zero to 48 hours with 1) an MR-positive bacteria and 2) a MR-negative bacteria. We know from the Exercise 5-2 Phenol Red Broth that all of the Unknown Bacteria Species ferment glucose with acid end products. Streptococcus pyogenes is MR negative and VP negative. S. pyogenes does not perform a mixed acid fermentation (purple dashed line). However, S. pyogenes does produce acid from glucose fermentation. Explain how the phosphate buffer in the media works to maintain a constant pH of 7 throughout the incubation even though S. pyogenes produces acid.
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. A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium? a. Name four categories that blood agar fits. Alpha hemolysis Beta hemolysis Gamma Alpha prime Name four differential reactions that TSIA shows. Observe figure 3.15. Suggest what causes the difference in growth pattern between nonmotile and motile bacteria. Explain what a medium that is both selective and differential does, using figure 3.18. a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? MSA Mannitol salt agar One that lives in the human stomach? Why are intestinal bacteria able to grow…
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