Here is the protocol for a UV-Vis spectrophotometer to detect water and chlorine-carbon. 1.Dissolve the water and chlorine-carbon compounds in a solvent, such as water. 2.Prepare a standard solution of known concentration that is similar to the sample being measured. 3.Calibrate the spectrophotometer using the standard solution. 4.Measure the absorbance of the sample using the spectrophotometer. 5.Calculate the concentration of the compounds in the sample using the calibration curve obtained from the standard solution. How is the spectrophotometer calibrated with standard solutions? When is the blank solution placed in the spectrophotmeter?

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UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or  ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.