analgesic drug sample was analyzed using TLC to determine its probable active ingredient. The compound may contain compound A, compound B, compound C, or compound D. Five spots were made on the TLC plate, of which four are for the standard compounds (A, B, C and D) and one for the test sample. The solvent was then allowed to pass through the TLC plate. The calculated Rf values of compound A, compound B, compound C, compound D, and the drug sample were 0.24, 0.61, 0.40, 0.89, 0.38, respectively. a. What could be the active ingredient

analgesic drug sample was analyzed using TLC to determine its probable active ingredient. The compound may contain compound A, compound B, compound C, or compound D. Five spots were made on the TLC plate, of which four are for the standard compounds (A, B, C and D) and one for the test sample. The solvent was then allowed to pass through the TLC plate. The calculated Rf values of compound A, compound B, compound C, compound D, and the drug sample were 0.24, 0.61, 0.40, 0.89, 0.38, respectively. a. What could be the active ingredient

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter89: Thin-layer Chromatography

Section: Chapter Questions

Problem 1P

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An analgesic drug sample was analyzed using TLC to determine its probable active ingredient. The compound may contain compound A, compound B, compound C, or compound D. Five spots were made on the TLC plate, of which four are for the standard compounds (A, B, C and D) and one for the test sample. The solvent was then allowed to pass through the TLC plate. The calculated Rf values of compound A, compound B, compound C, compound D, and the drug sample were 0.24, 0.61, 0.40, 0.89, 0.38, respectively. a. What could be the active ingredient in the analgesic drug sample? Why?
A student spots an unknown sample on a TLC plate and develops it in dichloromethane solvent. Only one spot, for which the Rf value is 0.95, is observed. Does this indicate that the unknown material is a pure compound? What can be done to verify the purity of the sample?
A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot, for which the Rf value is 0.05, is observed. Is the un-known material a pure compound? What can be done to verify the purity of the sample?
As solvent polarity increases, the Retention Factor (Rf) of compounds on the TLC plate increases. Group of answer choices True False
A student places an unknown sample on a thin-layer chromatography plate and develops it in dichloromethane as a solvent. Only one spot whose Rf is 0.98 is observed. Does this result indicate that the unknown material is a pure compound? If not a pure compound, suggest an alternate procedure to verify the purity of the sample.
Your manager has asked you to develop an analysis method for an ingredient in a perfume product being developed for the cosmetic industry. The ingredient has a high vapor pressure. The lab has instrumental analysis equipment. The manager specifies that in addition to the method being accurate, precise, and specific for the ingredient, it should be inexpensive and generate minimal chemical waste. Select the most promising analysis technique based on the information provided. a) Gas Chromatography (GC) with Flame Ionization Detection b) Liquid-liquid Extraction with Infrared Spectroscopy c) Gel Electrophoresis d) HPLC with Evaporative Light Scattering Detection
In a normal phase chromatography set up, the component that yields the highest Rf value is likely to be _____. Group of answer choices Both polarity Cannot be determined Polar Non-polar
The following are the uses of TLC except Choices: Determine the number of components in a sample Detect a given compound or compounds in a very crude mixture Determine the concentration of certain components of the mixture Find suitable conditions prior to running a column chromatogram None of the above
Why are some of the detectors listed unsuitable for HPLC?
An HPLC method was developed for the separation and determination of ibuprofen in blood samples. A 3.00 mL blood sample dissolved in 70.00 mL solvent was prepared and 10.0 mL was injected into the HPLC. Standard ibuprofen solutions were also run.Calculate the ibuprofen present in the 3.00 mL blood sample The following data were obtained. Table po itoo: Ibuprofen concentration, mg/ml0.50 1.0 2.0 3.0 Blood sample Peak area (Right column)5.29.617.419.911.4
106.54 mg of an impure sample of the drug was diluted in 100 ml and injected in a HPLC, the AUC of the major peak in the chromatogram integrated 1140 units. A standard solution containing 1.13 mg/ml of this drug was then injected in the same HPLC and the resulting peak integrated 1912 units. What is the %w/w of purity for this sample?(One decimal place, no units)
You must separate three different compounds with reversed phase HPLC. Compound 1 is an acid (pKa = 4.3), compound 2 is neutral compounds and compound 3 is a base (pKa = 9.6). As eluent, use 30/70 (v / v)% methanol / pH 3.0 formic acid buffer. The retention time and the top width at the base of the top for the three components are presented below. You can assume the dead time to 1.2 minutes in your calculations.Compound Retention time [minutes] Peak width [seconds] 1 10.1 282 5.0 143 6.1 17a) Calculate the efficiency (number of theoretical bases) for component 1.b) Calculate the selectivity and resolution between components 1 and 2.c) When you increase the pH of your eluent to pH 6, how will the retention be affected (increase, decreaseor unchanged) for the different associations (1, 2 and 3) ?, justify your answer.d) How retention factors are generally affected by an increase in the proportion organicallymobile phase solvents for reversed phase HPLC separations ?, justify your…