How can you assess dominance and mapping with only one mutant?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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How can you assess dominance and mapping with only one mutant?

Using a new model lizard with a wild-type strain and genome sequence, you identify a
single mutant (the first mutant strain in this organism) that is defective in scale generation.
Transgenesis and CRISPR-Cas9 genome editing are possible in this organism. What
order of steps (shown below) will be the best approach to identify the gene mutated in this
strain?
(1) Sequencing of wild-type and mutant strain genomes
(2) Mapping
(3) Complementation
(4) Transgenic rescue of the mutant phenotype by the wild-type gene
(5) Assess dominance
(6) CRISPR of the gene in the wild-type strain
A. (1), (2), (3), (4), (5), (6)
B. (2), (6) - Skip other steps
C. (5), (2), (1), (6), (4) - Skip step 3
D. (5), (3), (2), (1), (6), (4)
E. (1), (6), (4) - Skip other steps
Close
Session ID: 517697
119
Responses
X
136
2022 Exam 2 #12
Transcribed Image Text:Using a new model lizard with a wild-type strain and genome sequence, you identify a single mutant (the first mutant strain in this organism) that is defective in scale generation. Transgenesis and CRISPR-Cas9 genome editing are possible in this organism. What order of steps (shown below) will be the best approach to identify the gene mutated in this strain? (1) Sequencing of wild-type and mutant strain genomes (2) Mapping (3) Complementation (4) Transgenic rescue of the mutant phenotype by the wild-type gene (5) Assess dominance (6) CRISPR of the gene in the wild-type strain A. (1), (2), (3), (4), (5), (6) B. (2), (6) - Skip other steps C. (5), (2), (1), (6), (4) - Skip step 3 D. (5), (3), (2), (1), (6), (4) E. (1), (6), (4) - Skip other steps Close Session ID: 517697 119 Responses X 136 2022 Exam 2 #12
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