Part I. Structure-Function Relationships in Genes 1. Consider the "two-line model" of a gene shown below - each line represents one strand of a DNA double helix, and the transcription start site is indicated as +1. Use the two-line models provided when answering the following questions. in in 3' 5' +1 in m 5' 3' Assume that you know RNA polymerase will move to the right during transcription. On the diagram above, do the following: • Label "upstream" and "downstream" on this gene • Label where you would find the promoter • Draw a box where you would expect to find the TATA box Draw a third line below the model representing the RNA transcript (label the ends!) • Label one of the DNA strands as the template strand

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter29: Transcription And The Regulation Of Gene Expression
Section: Chapter Questions
Problem 16P
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please help with question 1 and 2.

Part I. Structure-Function Relationships in Genes
1. Consider the "two-line model" of a gene shown below - each line represents one strand of
a DNA double helix, and the transcription start site is indicated as +1. Use the two-line
models provided when answering the following questions.
3'
5'
Assume that you know RNA polymerase will move to the right during transcription. On
the diagram above, do the following:
• Label "upstream" and "downstream" on this gene
+1
• Label where you would find the promoter
• Draw a box where you would expect to find the TATA box
• Draw a third line below the model representing the RNA transcript (label the ends!)
• Label one of the DNA strands as the template strand
៦.
2. Now, let's try that again! This time assume that you know RNA polymerase will move to the
left during transcription. Repeat the same tasks as before on the diagram below:
3'
5'
3'
5'
+1
3. In your own words, describe how you can determine on which side of +1 the RNA
transcript will be made, and how you determine the template strand?
in in
Transcribed Image Text:Part I. Structure-Function Relationships in Genes 1. Consider the "two-line model" of a gene shown below - each line represents one strand of a DNA double helix, and the transcription start site is indicated as +1. Use the two-line models provided when answering the following questions. 3' 5' Assume that you know RNA polymerase will move to the right during transcription. On the diagram above, do the following: • Label "upstream" and "downstream" on this gene +1 • Label where you would find the promoter • Draw a box where you would expect to find the TATA box • Draw a third line below the model representing the RNA transcript (label the ends!) • Label one of the DNA strands as the template strand ៦. 2. Now, let's try that again! This time assume that you know RNA polymerase will move to the left during transcription. Repeat the same tasks as before on the diagram below: 3' 5' 3' 5' +1 3. In your own words, describe how you can determine on which side of +1 the RNA transcript will be made, and how you determine the template strand? in in
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