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- In what ways can you use aseptic technique principles in your daily life to preserve a healthy lifestyle, particularly in light of the CoVID-19 pandemic? Explain in four to five sentences.In a mixed infection assay between h+r+ and hr phage, where h and r are two genes that govern plaque morphology, which of the following do you expect to observe? only h+r+ and hr only h+r and hr+ only h+r+ only hr All four types: h+r+, hr, h+r, hr+In ELISPOT Assay, explain the importance of adding stimulus to the 96-well plate during the incubation of Peripheral Blood mononuclear cells
- Bacterial mutants can be classified on the basis of the method, Which is those methods?The strain of λ phage t is cI857. That tells you that the cI DNA segment is disabled by a specific mutation. What is the exact genetic change in cI857? What specific property of the cI gene product does this mutation change, and how does this help titering for a plaque assay?Briefly describe multi locus sequence typing (MLST) as it relates to the identification of Listeria monocytogenes.
- Does endogenous contamination/component in crude extracted protein make interfere to transfer protein on PVDF membrane for western blot?You decide to use a plaque assay with a human lung epithelial cell line to compare the effectiveness of the drug. The results of the plaque assay for one strain of influenza are shown below. Based on these results, what can you conclude about the relative effectiveness of WJ379 at 1 mM compared to oseltamivir at 1 mM? Briefly explain your rationale.During the first successful gene therapy trial in which Ashanti DeSilva was treated for SCID, did the therapeutic gene delivered to Ashanti replace the defective copy of the ADA gene? Why were white blood cells chosen as the targets for the therapeutic gene?
- A microbial geneticist isolates a new mutation in E. coliand wishes to map its chromosomal location. She usesinterrupted-mating experiments with Hfr strains andgeneralized-transduction experiments with phage P1.Explain why each technique, by itself, is insufficient foraccurate mapping.By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:Explain how these results are consistent with the idea that thebacterial chromosome is circular?describe how the exercise of streaking a loop full of lytic T4-phage suspension over a petri dish with E. coli B, Enterobacter aerogenes and Staphylococcus aureus demonstrates the principle of phage typing.