how to read this table? like for example, just by looking at the table, it was said that leptin has 16 kda with 15% gel. How did they able to determine that leptin needs 15% gel?

Biology (MindTap Course List)
11th Edition
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Chapter3: The Chemistry Of Life: Organic Compounds
Section: Chapter Questions
Problem 9TYU: Which of the following levels of protein structure may be affected by hydrogen bonding? (a) primary...
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how to read this table? like for example, just by looking at the table, it was said that leptin has 16 kda with 15% gel. How did they able to determine that leptin needs 15% gel?

The separation of molecules within a gel is determined by the relative size of the pores
formed within the gel. Thus, the gel percentage depends on the size of your protein of
interest.
Protein size (kDa)
Gel percentage (%)
4-40
20
12-45
15
10-70
12.5
15-100
10
25-200
8
Transcribed Image Text:The separation of molecules within a gel is determined by the relative size of the pores formed within the gel. Thus, the gel percentage depends on the size of your protein of interest. Protein size (kDa) Gel percentage (%) 4-40 20 12-45 15 10-70 12.5 15-100 10 25-200 8
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