Determine the OPTIMUM pH for the enzyme reaction using the software simulation: (HINT: Run the enzyme reaction at the OPTIMUM temperature for the most accurate results) 2 03 04 05 06 07 08 09 10 11 12

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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QUESTION 1
Determine the OPTIMUM pH for the enzyme reaction using the software simulation:
(HINT: Run the enzyme reaction at the OPTIMUM temperature for the most accurate results)
02 03 04 05 06 07 08 09 10 11 12
QUESTION 2
Determine the OPTIMUM temperature for the enzyme reaction using the software simulation:
(HINT: Run the enzyme reaction at the OPTIMUM pH for the most accurate results)
O 27 °C 28 °C 29 °C O O
O 33 34 O
O
O 43 44 45
°C
QUESTION 3
Calculate the Km of the enzyme without any inhibitor when using 6U of Alkaline phosphatase per reaction under the OPTIMAL conditions for pH and
temperature to obtain the raw data.
(In mM)
ㅎㅇ
O
O
C
Transcribed Image Text:QUESTION 1 Determine the OPTIMUM pH for the enzyme reaction using the software simulation: (HINT: Run the enzyme reaction at the OPTIMUM temperature for the most accurate results) 02 03 04 05 06 07 08 09 10 11 12 QUESTION 2 Determine the OPTIMUM temperature for the enzyme reaction using the software simulation: (HINT: Run the enzyme reaction at the OPTIMUM pH for the most accurate results) O 27 °C 28 °C 29 °C O O O 33 34 O O O 43 44 45 °C QUESTION 3 Calculate the Km of the enzyme without any inhibitor when using 6U of Alkaline phosphatase per reaction under the OPTIMAL conditions for pH and temperature to obtain the raw data. (In mM) ㅎㅇ O O C
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