How would a decrease in the wavelength of light affect the resolution power of a bright-field microscope?

Q: A calibration plot for a particular absorbing species is constructed according to the Beer's…

A: The expression of beer's law is shown below:A =ε c l where; A = absorbance c = concentration l =…

Q: What are the molecular processes that occur when (a) infrared, (b) x-ray, and (c) ultraviolet…

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Q: What are the side effects of X-ray irradiation?

A: The electronic transitions taking place within the atoms between different energy levels generate a…

Q: UV-Vis spectrophotometry is used to determine copper. Aside from UV-Vis spectrophotometry what other…

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Q: A researcher finds that a sample of an unknown solution, when placed in a 1.00 cm wide cuvette,…

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Q: What is the significance of the Amax of a spectrum? What are the other uses of the…

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Q: 3. A 2.50×104M solution of a dye has an absorbance of 0.680 when measured in a 1.00 cm cell at 625…

A: The relation between absorbance and transmittance is given by A=2-logT     or A=-logT where A=…

Q: Spectrum 99 97 95 93 91 89 87 85 83 81 79 77 4000 3500 3000 2500 2000 1500 1000 500 Wave Number…

A: IR provides the information about the functional groups of unknown.

Q: What does the absorbance of a protein at 280 nm reflect and how does that information help you…

A: In UV-Visible spectroscopy light is passed through the sample and if the compound to be analyzed…

Q: Define refractive index of liquids . How we measure the refractive index of liquid by abbe's…

A: The refractive index of a material is a  dimensionless number that describes how fast light travels…

Q: Do the wavelengths (at the highest % transmittance) match to the color of the solution in the…

A: In the highest transmittance, the weave length of the light does not math with color with solutions…

Q: .A diffraction grating has 410 lines per mm. If light of wavelength 560 nm is sent through this…

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Q: 3. In molecular absorption spectrophotometry, the relationship between transmittance and…

A: absorption spectrophotometry It is used for analyzing the Concentration of various sample by based…

Q: Is standardization against primary standard required for polarography?

A: A question based on electroanalytical methods that is to be accomplished.

Q: Plot a graph of absorbance A vs. wavelength λ and determine the λ max (maximum wavelength)

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Q: If a solution has an absorbance value of 0.325 at a wavelength of 450 nm, what is the percent…

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Q: Why should absorbance be less than 2?  Please explain in terms of light transmittance

A: Absorbance A also called the optical density , is the quantity of light absorbed by a solution.

Q: Explain what is meant by signal in instrumental analysis.

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Q: In a double-beam- in-time instrument a beam - splitter is required to split the radiation from the…

A: The basic principle of spectrophotometer is to major the intensity of light absorbed at different…

Q: Lambert–Beer law A transparent solution will have a transmittance of _____ (100% / 0% / 50%) . A…

A: According to this law the absorbance of a solution is directly proportional to the concentration of…

Q: Indicate the relationship of proportionality that exists between: a) concentration and absorbance…

A: Applying concept of Beers law.

Q: 1. What is the absorbance of a substance that has a transmittance of 68%?

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Q: Convert the following transmittance or % transmittance to absorbance.         a. 0.305     b.…

A: The transmittance of a solution can be described as the ratio of the intensity of the light…

Q: A student uses visible spectrophotometry to determine the concentration of CoCl2(aq)CoCl2(aq) in a…

A: This question asks one to find the concentration of a solution where CoCl2(aq) as the solute and…

Q: In optical analysis, what are the methods used to calculate the concentration of a solution from the…

A: Solution: Colorimetry is the method of calculating the concentration of a coloured compound present…

Q: what are the applications of Atomic Force Microscopy and explain each

A: Atomic Force Microscopy is a technique which is used to image different type of surfaces like…

Q: The refractive index of a solution composd of a subtance with a molecular formula of C6H602 with a…

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Q: Project 2: Food Dye Spectroscopy   What is the relationship between absorbance and transmittance?

A: Absorbance is the measure of capacity of a sample to absorb light of a specified wavelength.…

Q: 22) A parallel beam of monochromatic light of wavelength 490nm is incident on a diffraction grating.…

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Q: Briefly describe how a phase-contrast microscope work and the kind of image that it produces. Give a…

A: Phase-contrast microscopy generates an image by using the different refractive indexes and densities…

Q: 6-what is difference between Transmittance and absorbance in spectroscopy?

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Q: The scale of a spectrophotometer extends from 1 to 100% T, what are the values of these two extremes…

A: We have to predict the values in terms of Absorbance.

Q: 4. (a) Calculate the Doppler width (as a fraction of the transition wavelength) for any kind of…

A: Given: Temperature = 25 oC = 298 K Molar mass of HCl = 36.45 g/mol Let the transition wavelength be…

Q: Express the absorbance of 0.66 in terms of percent transmittance. A. 66% В. 22% С. 78% D. 34% Е. 33%

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Q: Beer’s law plot is prepared by plotting absorbance vs molar concentration of the absorbing species.…

A: The units for the above given as

Q: The scale of a spectrophotometer extends from 1 to 100% T, what are the values of these two extremes…

A: A = -log(T) A = log(1/T) A = log[100/T(%)] A = 2-log[T(%)]

Q: The absorbance of an ionic aqueous solution is 0.387. What is the ratio of intensities of the…

A: The absorbance of a solution can be expressed as the negative logarithm of the ratio of incident…

Q: A 0.1 cm cuvette containing sample solution is irradiated with monochromatic light of intensity lo.…

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Q: 22.6, t 25.1, t n the highlighted area to zoom. Click again to zoom back out.

A: From C-13 NMR we can identify different types of Carbon with their electronic environment.

Q: How will you use a solution of known concentration of one of the food dyes to determine the…

A: In UV-visible spectrum, different types of electronic transitions taking place between the HOMO and…

Q: The refractive index of a compound is 1.622 for 643 nm light. Its mass density at 20 °C is 2.99 g…

A: Given data contains, Refractive index of compound is 1.622. The wavelength given is 643 nm. The mass…

Q: What happens to the result of a thick sample subjected to Micro X-ray Fluorescence? cannot map…

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Q: What is the diagonal effect? How does it explain the similarity in chemistry between, for example,…

A: Diagonal effect is the relationship between two adjacent elements which are located in second and…

Q: Which of the ff. angles is used for absorbance and transmittance measurements? 180 degrees 135…

A: A multiple choice question based on analytical process that is to be accomplished.

Q: The frequency range of infrared light used in an IR spectrometer is 1.2x1014 Hz to 1.2x1013 Hz. What…

A: Given, The frequency range of infrared light used in an IR spectrometer is 1.2x1014 Hz to 1.2x1013…

Q: Food dye spectroscopy  2. At what wavelengths should the readings be taken? What instrumentation…

A: Dyes are coloured substances so they are generally analysed in the visible region (400 nm to 800…

Q: Of particle size techniques, which technique can identify the 3-D dimensions of the particles? The…

A: A multiple choice question based on introduction to chemistry that is to be accomplished.

Q: A solution has a %T value of 48.2% at 600 nm. What is the absorbance of this solution at this…

A: The relation between absorbance (A) and percent transmittance (%T) is,