How would I calculate the amount of each component in each solution? Stock solutions provided: 0.5 M EDTA, pH 8 10% bromophenol blue 10% xylene cyanol Sucrose powder Tris powder Glacial acetic acid Solution A: 50 mL of 50x Tris Acetate EDTA (TAE) Buffer 2M Tris Base 50mM EDTA, pH 8 11.4% (v/v) glacial acetic acid Solution B: 1L of 1x TAE Buffer 40mM Tris-acetate 1mM EDTA Solution C: 5mL of 10x Loading Dye 0.25% (w/v) bromophenol blue 0.25% (w/v) xylene cyanol 66.5% (w/v) sucrose 100mM EDTA, pH 8
How would I calculate the amount of each component in each solution? Stock solutions provided: 0.5 M EDTA, pH 8 10% bromophenol blue 10% xylene cyanol Sucrose powder Tris powder Glacial acetic acid Solution A: 50 mL of 50x Tris Acetate EDTA (TAE) Buffer 2M Tris Base 50mM EDTA, pH 8 11.4% (v/v) glacial acetic acid Solution B: 1L of 1x TAE Buffer 40mM Tris-acetate 1mM EDTA Solution C: 5mL of 10x Loading Dye 0.25% (w/v) bromophenol blue 0.25% (w/v) xylene cyanol 66.5% (w/v) sucrose 100mM EDTA, pH 8
Chapter17: Introduction To Iv Therapy
Section: Chapter Questions
Problem 3.6P
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How would I calculate the amount of each component in each solution?
Stock solutions provided:
- 0.5 M EDTA, pH 8
- 10% bromophenol blue
- 10% xylene cyanol
- Sucrose powder
- Tris powder
- Glacial acetic acid
Solution A: 50 mL of 50x Tris Acetate EDTA (TAE) Buffer
- 2M Tris Base
- 50mM EDTA, pH 8
- 11.4% (v/v) glacial acetic acid
Solution B: 1L of 1x TAE Buffer
- 40mM Tris-acetate
- 1mM EDTA
Solution C: 5mL of 10x Loading Dye
- 0.25% (w/v) bromophenol blue
- 0.25% (w/v) xylene cyanol
- 66.5% (w/v) sucrose
- 100mM EDTA, pH 8
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